Analysis of the mechanism of nonsusceptibility to the densonucleosis virus (densovirus) type-2 in the silkworm, Bombyx mori, using a genetic mosaic

Abe, H.; Yokoyama, T.; Oshiki, T.; Kobayashi, M.

Journal of Sericultural Science of Japan 62(1): 38-44


Accession: 008158224

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Bombyx mori densonucleosis virus type-2 (BmDNV-2) multiplies in the nuclei of the columnar cells of the midgut epithelium of the silkworm larva, Bombyx mori. Some silkworm strains are genetically nonsusceptible (completely resistant) to BmDNV-2 infection even after the administration of a high dose of BmDNV-2. This nonsuscoeptibility to BmDV-2 is controlled by a recessive gene (nsd-2; nonsusceptibility to BmDNV-2). In order to investigate whether the nonsusceptibility controlled by the nsd-2 gene is determined at the whole body level or cellular level, susceptible non-susceptible bilateral mosaic larvae were produced using the herediatary mosaic strain (mo) and we administered BmDNV-2. By immunoperoxidase staining of the larval body section, five mosaic larvae displaying midgut mosaicism were detected in two areas, infected (nsd-2/+) and noninfected (nsd-2/nsd-2), respectively. These results indicate that, the nonsusceptibility to BmDNV-2 is determined by a single gene at a single cellular level.