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Analysis of the reaction products of N-(O,O-diisopropyl)phosphorylthreonine with uridine by capillary zone electrophoresis with diode array detection and capillary electrophoresis-mass spectrometry



Analysis of the reaction products of N-(O,O-diisopropyl)phosphorylthreonine with uridine by capillary zone electrophoresis with diode array detection and capillary electrophoresis-mass spectrometry



Journal of Chromatography A 745(1-2): 263-272



The reaction products of N-(O,O-diisopropyl)phosphorylthreonine with uridine were analyzed by capillary zone electrophoresis with diode array detection using 50 mM boric acid buffer at pH 8.5. Peaks in the electropherograms were identified by the on-line UV spectra as well as some authentic standards. The influence of buffer composition and capillary coating on separations were studied. The products were also analyzed by capillary electrophoresis-mass spectrometry with a sheath-flow electrospray ionization interface. Identification was achieved by reconstructed ion electropherograms. The existence of nucleotides, homooligonucleotides and their derivatives in the products was confirmed by both methods, which supports a hypothesis for the origin of life.

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Accession: 008158511

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DOI: 10.1016/0021-9673(96)00400-1



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