+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Analysis of yeast trimethylguanosine-capped RNAs by Midwestern blotting



Analysis of yeast trimethylguanosine-capped RNAs by Midwestern blotting



Gene (amsterdam). 182(1-2): 89-96



Immuno-detection by 'Midwestern' blotting provides a simple way to identify trimethylguanosine (TMG) capped RNAs. With this technique, over 20 bands are observed when total cellular RNA from Saccharomyces cerevisiae is transferred to a nylon membrane and probed with anti-TMG antibodies. Most, if not all, species known to contain a TMG cap are detected by this method. Only TMG-capped RNAs are detected on Midwestern blots unlike anti-TMG immunoprecipitates. Midwestern blotting is a useful alternative to immunoprecipitation and Northern analysis and may prove to be a better method for determining the relative abundance of capped RNAs. The blots can be reprobed multiple times with labeled antisense oligonucleotides to determine the identity of any TMG-capped species for which the primary sequence or a clone is available. This dual detection capability provides a powerful tool for the analysis of TMG-capped snRNAs and snoRNAs.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008159270

Download citation: RISBibTeXText

PMID: 8982072

DOI: 10.1016/s0378-1119(96)00519-7


Related references

A library of trimethylguanosine-capped small RNAs in Physarum polycephalum. Gene 54(1): 93, 1987

Trimethylguanosine capped rnas identified in a prokaryotic cyanobacterium synechococcus leopoliensis. Journal of Cell Biology 109(4 Part 2): 38A, 1989

Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways. Journal of Biological Chemistry 283(46): 31706-31718, 2008

Distribution of 5'-trimethylguanosine capped small nuclear RNAs in extrachromosomal oocyte nuclear domains of the laboratory insect, Tribolium castaneum. Doklady Biological Sciences 444: 198-201, 2012

The nematode eukaryotic translation initiation factor 4E/G complex works with a trans-spliced leader stem-loop to enable efficient translation of trimethylguanosine-capped RNAs. Molecular and Cellular Biology 30(8): 1958-1970, 2010

Partial characterization of the trimethylguanosine capped small rna in physarum polycephalum. Federation Proceedings 45(6): 1704, 1986

Trimethylguanosine nucleoside inhibits cross-linking between Snurportin 1 and m3G-CAPPED U1 snRNA. Nucleosides, Nucleotides & Nucleic Acids 25(8): 909-923, 2006

Screening a physarum complementary dna library for trimethylguanosine capped small rna species. Federation Proceedings: 2013, 1987

Construction and screening of a complementary dna library for trimethylguanosine capped small rna in physarum polycephalum. Federation Proceedings 45(6): 1704, 1986

RNA B is the major nucleolar trimethylguanosine-capped small nuclear RNA associated with fibrillarin and pre-rRNAs in Trypanosoma brucei. Molecular and Cellular Biology 13(1): 144-154, 1993

A monoclonal antibody against 2 2 7 trimethylguanosine that reacts with intact class u small nuclear ribonucleoproteins as well as with 7 methylguanosine capped rna. European Journal of Biochemistry 168(2): 461-468, 1987

Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs. Proceedings of the National Academy of Sciences of the United States of America 107(33): 14787-14792, 2010

Antibodies elicited by N2,N2-7-trimethylguanosine react with small nuclear RNAs and ribonucleoproteins. Journal of Biological Chemistry 258(7): 4636-4640, 1983

Novel scleroderma autoantibodies to the u11 small nuclear ribonucleoprotein particle snrnp and the trimethylguanosine cap of u rnas. Clinical Research 40(2): 307A, 1992

The 5 ends of influenza viral messenger RNAs are donated by capped cellular RNAs. Trends in Biochemical Sciences 6: 7-10, 1981