+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Angiogenesis-directed implantation of genetically modified endothelial cells in mice

Angiogenesis-directed implantation of genetically modified endothelial cells in mice

Cancer Research 55(11): 2240-2244

By virtue of their location within blood vessels and their ability to express foreign genes, endothelial cells are attractive vehicles for the delivery of therapeutic molecules in vivo. We wished to determine whether i.v.-injected, genetically modified endothelial cells can become incorporated into sites of active angiogenesis in vivo. To do so, we studied the fate of i.v.-injected, lacZ-expressing human umbilical vein endothelial cells in athymic nude mice bearing lethally irradiated NIH 3T3 murine fibroblast cells transfected with a sp-hst/KS3:fibroblast growth factor-1 chimera that forces the secretion of the angiogenic protein, fibroblast growth factor-1. Following i.v. injection, lacZ-labeled human umbilical vein endothelial cells accumulated at sites of fibroblast growth factor-1-induced angiogenesis, persisting for at least 4 weeks. These results suggest that i.v.-administered, genetically modified endothelial cells can migrate into and survive within an angiogenic site. This strategy may be useful for delivery of therapeutic molecules to sites of pathological angiogenesis during tumor metastasis.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 008162051

Download citation: RISBibTeXText

PMID: 7538897

Related references

Angiogenesis-directed implantation of genetically-modified endothelial cells at sites of pulmonary metastases. Proceedings of the American Association for Cancer Research Annual Meeting 37: 346, 1996

Implantation of vascular grafts lined with genetically modified endothelial cells. Science 244(4910): 1344-1346, 1989

Genetically modified endothelial cells administered intravenously are incorporated into sites of active angiogenesis. Journal of Cellular Biochemistry Suppl. (16 Part F): 50, 1992

Implantation of genetically modified endothelial cells. A new promising technique for gene therapy. Sheng Li Ke Xue Jin Zhan 23(2): 162-163, 1992

Augmentation of Therapeutic Angiogenesis Using Genetically Modified Human Endothelial Progenitor Cells with Altered Glycogen Synthase Kinase-3b Activity. The Journal of Biological Chemistry 279(47): 430-8, 2004

Augmentation of therapeutic angiogenesis using genetically modified human endothelial progenitor cells with altered glycogen synthase kinase-3beta activity. Journal of Biological Chemistry 279(47): 49430-8, 2004

Endothelial cells genetically modified to produce gamma interferon inhibit tumor growth in SCID mice. Cancer Gene Therapy 4(6 CONF Suppl. ): S54, 1997

In vivo enhancement of angiogenesis by adenoviral transfer of HIF-1alpha-modified endothelial progenitor cells (Ad-HIF-1alpha-modified EPC for angiogenesis). International Journal of Biochemistry and Cell Biology 40(10): 2284-2295, 2008

Therapeutic angiogenesis using genetically engineered human endothelial cells. Journal of Controlled Release 160(3): 515-524, 2012

NTP report on the toxicology studies of dicyclohexylcarbodiimide (CAS No. 538-75-0) in F344/N rats, B6C3F 1 mice, and genetically modified (FVB Tg.AC hemizygous) mice and carcinogenicity study of dicyclohexylcarbodiimide in genetically modified [B6.129-Trp53 tm1Brd (N5) haploinsufficient] mice (dermal studies). National Toxicology Program Genetically Modified Model Report: 1-138, 2008

Implantation of genetically modified mesencephalic fetal cells into the rat striatum. Brain Research Bulletin 29(1): 81-93, 1992

Genetically modified human umbilical cord blood cells expressing vascular endothelial growth factor and fibroblast growth factor 2 differentiate into glial cells after transplantation into amyotrophic lateral sclerosis transgenic mice. Experimental Biology and Medicine 236(1): 91-98, 2011

Antinociception following implantation of AtT-20 and genetically modified AtT-20/hENK cells in rat spinal cord. Journal of Neural Transplantation and Plasticity 4(1): 15-26, 1993

Implantation of genetically modified cells into the rat brain an approach to restoration of cns functions. Beaudet, A L , R Mulligan And I M Verma (Ed ) Ucla (University Of California Los Angeles) Symposia on Molecular And Cellular Biology New Series, Vol 87 Gene Transfer And Gene Therapy; Tamarron, Colorado, Usa, February 6-12, 1988 Xxii+441p Alan R Liss, Inc : New York, New York, Usa Illus 409-416, 1989

Directed differentiation and characterization of genetically modified embryonic stem cells for therapy. Methods in Molecular Biology 329: 471-484, 2006