+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Angiotensin II-induced fluid phase endocytosis in human cerebromicrovascular endothelial cells is regulated by the inositol-phosphate signaling pathway



Angiotensin II-induced fluid phase endocytosis in human cerebromicrovascular endothelial cells is regulated by the inositol-phosphate signaling pathway



Journal of Cellular Physiology 169(3): 455-467



The involvement of the early signaling messengers, inositol tris-phosphate (IP3), intracellular calcium, [Ca2+]i, and protein kinase C (PKC), in angiotensin II (AII)-induced fluid phase endocytosis was investigated in human brain capillary and microvascular endothelial cells (HCEC). ALL (0.01-10 microM) stimulated the uptake of Lucifer yellow CH, an inert dye used as a marker for fluid phase endocytosis, in HCEC by 50-230%. AII also triggered a fast accumulation of IP3 and a rapid increase in [Ca2+]i in cells loaded with the Ca(2+)-responsive fluorescent dye fura-2. The prompt AII-induced [Ca2+]i spike was not affected by incubating HCEC in Ca(2+)-free medium containing 2 mM EGTA or by pretreating the cultures with the Ca2+ channel blockers, methoxyverapamil (D600; 50 microM), nickel (1 mM), or lanthanum (1 mM), suggesting that the activation of AII receptors on HCEC triggers the release of Ca2+ from intracellular stores. The AII-triggered increases in IP3, [Ca2+]i, and Lucifer yellow uptake were inhibited by the nonselective AII receptor antagonist, Sar1, Val5, Ala8-AII (SVA-AII), and by the phospholipase C (PLC) inhibitors, neomycin and U-73122. By contrast, the protein kinase C (PKC) inhibitors, staurosporine and calphostin C, failed to affect any of these AII-induced events. This study demonstrates that increased fluid phase endocytotosis induced by AII in human brain capillary endothelium, an event thought to be linked to the observed increases in blood-brain barrier permeability in acute hypertension, is likely dependent on PLC-mediated changes in [Ca2+]i and independent of PKC.

(PDF emailed within 0-6 h: $19.90)

Accession: 008162959

Download citation: RISBibTeXText

PMID: 8952695

DOI: 10.1002/(sici)1097-4652(199612)169:3<455::aid-jcp6>3.0.co;2-n


Related references

Impairment of erbB1 receptor and fluid-phase endocytosis and associated mitogenic signaling by inositol hexaphosphate in human prostate carcinoma DU145 cells. Carcinogenesis 21(12): 2225-2235, 2001

Impairment of erbB1 receptor and fluid-phase endocytosis and associated mitogenic signaling by inositol hexaphosphate in human prostate carcinoma Du145 cells. Carcinogenesis 21(12): 2225-2235, 2000

PI3K/Akt/uncoupling protein 2 signaling pathway may be involved in cell senescence and apoptosis induced by angiotensin II in human vascular endothelial cells. Molecular Biology Reports 41(10): 6931-6937, 2015

Mitochondrial signaling in hydrogen peroxide-induced apoptosis in human umbilical vein endothelial cells Role of the pentose phosphate pathway. Faseb Journal. 15(5): A940, Ch 8, 2001

Further intracellular proteins and signaling pathways regulated by angiotensin-(1-7) in human endothelial cells. Data in Brief 10: 354-363, 2016

Distinct expression of receptors linked to Ca-2+ signaling in rat and human cerebromicrovascular endothelial cells. Society for Neuroscience Abstracts 22(1-3): 771, 1996

Identification of intracellular proteins and signaling pathways in human endothelial cells regulated by angiotensin-(1-7). Journal of Proteomics 130: 129-139, 2016

Reactive oxygen species mediate shear stress-induced fluid-phase endocytosis in vascular endothelial cells. Free Radical Research 40(2): 167-174, 2006

Angiotensin peptide regulation of fluid phase endocytosis in brain microvessel endothelial cell monolayers. Journal of Cerebral Blood Flow & Metabolism 10(6): 827-834, 1990

Bradykinin-induced Ca(2+)-influx into cultured aortic endothelial cells is not regulated by inositol 1,4,5-trisphosphate or inositol 1,3,4,5-tetrakisphosphate. Second Messengers and Phosphoproteins 13(4): 187-197, 1991

Bradykinin induced calcium influx into cultured aortic endothelial cells is not regulated by inositol 1 4 5 trisphosphate or inositol 1 3 4 5 tetrakisphosphate. Second Messengers & Phosphoproteins 13(4): 187-197, 1991

Mitochondrial ROS-K+ channel signaling pathway regulated secretion of human pulmonary artery endothelial cells. Free Radical Research 46(12): 1437-1445, 2013

Inhibition of angiotensin II-induced contraction of human airway smooth muscle cells by angiotensin-(1-7) via downregulation of the RhoA/ROCK2 signaling pathway. International Journal of Molecular Medicine 30(4): 811-818, 2013

Inhibition of CD40L induced E-selectin expression in endothelial cells through extracellular signal-regulated kinase signaling pathway. Zhonghua Yi Xue Za Zhi 88(48): 3432-3435, 2009

Inhibition of angiotensin II-induced inositol phosphate production by triacid nonpeptide antagonists in CHO cells expressing human AT1 receptors. Pharmaceutical Research 17(12): 1482-1488, 2001