+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antibodies against 1D, a recombinant 64-kDa membrane protein, are associated with ophthalmopathy in patients with thyroid autoimmunity



Antibodies against 1D, a recombinant 64-kDa membrane protein, are associated with ophthalmopathy in patients with thyroid autoimmunity



Clinical Immunology & Immunopathology. 70(3): 225-233



We have tested for serum antibodies reactive with 1D, a recombinant 65-kDa human thyroid protein which is also expressed in eye muscle, in patients with thyroid autoimmunity and ophthalmopathy by immunofluorescence and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. We also measured antibodies to a 64-kDa pig eye muscle membrane protein which is identified by SDS-PAGE and Western blotting, correlating the two reactivities. While antibodies to 1D, expressed in Chinese hamster ovary (CHO) cell membrane, were detected in approximately 40% of patients with ophthalmopathy, in both tests the greatest prevalence, by immunofluorescence, 73%, was demonstrated in patients with Graves' hyperthyroidism without clinically evident eye disease, although only 50% of these patients were positive in immunoblotting. When the two tests for anti-1D antibodies were compared, immunofluorescence appeared to be the more specific and immunoblotting appeared to be the more sensitive. The greatest prevalence of antibodies reactive with a 64-kDa pig eye muscle protein, 71%, was in patients with TAO of less than 1 year duration; tests were positive in 49% of patients with more chronic ophthalmopathy and in 50% of patients with Graves' hyperthyroidism without evident eye disease. Antibodies reactive with 1D were detected in 17% of normals by immunofluorescence and 24% by immunoblots, while antibodies reactive with the 64-kDa pig eye muscle protein were detected in only 10% of the normal subjects tested. Lesser prevalences of antibodies to the two 64-kDa proteins in patients with established eye disease suggest that such antibodies may be an early abnormality in patients with Graves' hyperthyroidism who are predisposed to develop ophthalmopathy. Although the association was not close, reactivity against 1D by immunoblotting, but not immunofluorescence, was significantly correlated with reactivity to a 64-kDa eye muscle membrane protein by immunoblotting. On the other hand, when sera containing antibodies reactive with both 1D and the 64-kDa eye muscle protein were incubated with CHO (1D) cell membrane, reactivity against 1D was absorbed while that against the eye muscle protein was not. The precise relationship between the two 64-kDa proteins can only be clarified by cloning the 64-kDa protein from an eye muscle expression library and comparing the sequences with those of 1D.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008169612

Download citation: RISBibTeXText

PMID: 8313659

DOI: 10.1006/clin.1994.1033


Related references

Antibodies reactive with an intracellular epitope of a recombinant 64 kDa thyroid and eye muscle protein in patients with thyroid autoimmunity and ophthalmopathy. Journal of Endocrinological Investigation 16(11): 863-868, 1993

Long-term follow-up of seven patients with ophthalmopathy not associated with thyroid autoimmunity: heterogeneity of autoimmune ophthalmopathy. Clinical Ophthalmology 6: 1063-1071, 2012

Antibodies in the serum of patients with autoimmune thyroid disorders react with a recombinant 98 amino acid fragment of a full length 64 kDa eye muscle membrane protein which is also expressed in the thyroid. Autoimmunity 13(2): 151-157, 1992

Significance of antibodies reactive with a 64 kDa eye muscle membrane antigen in patients with thyroid autoimmunity. Thyroid 2(3): 197-202, 1992

Prevalence and significance of antibodies reactive with eye muscle membrane antigens in sera from patients with Graves' ophthalmopathy and other thyroid and nonthyroid diseases. Thyroid 8(2): 167-174, 1998

Eye muscle membrane reactive antibodies are not detected in the serum or immunoglobulin fraction of patients with thyroid-associated ophthalmopathy using an ELISA and crude membranes. Autoimmunity 7(1): 33-40, 1990

Antibodies reactive with eye muscle and thyroid membrane antigens in early and late thyroid associated ophthalmopathy. Nagataki, S, Mori, T, Torizuka, K International Congress Series; 80 years of Hashimoto disease 649-652, 1993

Isotype and immunoglobulin subclass distribution of eye muscle membrane reactive antibodies in the serum of patients with thyroid-associated ophthalmopathy as detected in Western blotting. Autoimmunity 10(1): 57-63, 1991

Titers of antibodies against thyroid membrane antigens in patients with Graves-Basedow disease and progressive infiltrative ophthalmopathy during treatment with glucocorticoids and retro-orbital tissue irradiation. Polski Tygodnik Lekarski 46(43-44): 824-826, 1991

Monoclonal antibodies to thyroid membrane antigens in the study of thyroid autoimmunity. Forti, G , M Serio And M B Lipsett (Ed ) Serono Symposia Publications From Raven Press, Vol 30 Monoclonal Antibodies: Basic Principles, Experimental And Clinical Applications in Endocrinology; Florence, Italy, Oct 2-4, 1985 Xx+325p Raven Press: New York, N Y , Usa Illus 245-252, 1986

Prevalence of antibodies reactive with a 64 kDa eye muscle membrane antigen in thyroid-associated ophthalmopathy. Thyroid 1(3): 207-213, 1991

T cell reactivity to a recombinant fragment of a novel 64 kDa protein in thyroid-associated ophthalmopathy. Nagataki, S, Mori, T, Torizuka, K International Congress Series; 80 years of Hashimoto disease 463-469, 1993

The role of long-acting thyroid stimulator and of thyroid autoimmunity in the pathogenesis of euthyroid endocrine ophthalmopathy. Acta Medica Academiae Scientiarum Hungaricae 28(2): 171-179, 1971

New assays for the measurement of serum antibodies reactive with eye muscle membrane antigens confirm their significance in thyroid-associated ophthalmopathy. Thyroid 5(3): 195-200, 1995