+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antibody response to a synthetic peptide derived from the human papillomavirus type 6/11 L2 protein in recurrent respiratory papillomatosis: Correlation between southern blot hybridization, polymerase chain reaction, and serology



Antibody response to a synthetic peptide derived from the human papillomavirus type 6/11 L2 protein in recurrent respiratory papillomatosis: Correlation between southern blot hybridization, polymerase chain reaction, and serology



Journal of Medical Virology 42(1): 52-59



Recurrent respiratory papillomatosis (RRP) is the most common benign tumour of the larynx, affecting both children and adults. We present a series of 25 patients, including 10 cases of juvenile multiple, 8 cases of adult solitary, and 7 cases of adult multiple RRP. Biopsy tissue from each patient was screened by Southern blot hybridization and polymerase chain reaction for the presence of human papillomavirus (HPV) DNA. Sera from patients and age- and sex-matched controls were tested for the presence of HPV-specific antibodies using a synthetic peptide derived from the minor capsid protein (L2) of HPV 6/11. By Southern blot hybridization and/or polymerase chain reaction, biopsies from all patients were positive for HPV 6/11 DNA. There was no difference in antibody response between cases and controls. Female cases and controls had significantly higher antibody titers than male subjects. A correlation was observed between the HPV-specific antibody level and the number of surgery-necessitating recurrences.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008170466

Download citation: RISBibTeXText

PMID: 8308521

DOI: 10.1002/jmv.1890420111


Related references

Determination of human neutrophil oxygen consumption rate by ctpo spin probe. Byulleten' Eksperimental'noi Biologii i Meditsiny 108(11): 561-563, 1989

Detection of human papillomavirus (HPV) type 16 DNA relative sequence in the tissue of laryngeal carcinoma. Zhonghua Er Bi Yan Hou Ke Za Zhi 26(3): 160, 1991

Human papillomavirus DNA in invasive carcinoma of the vagina. Obstetrics and Gynecology 76(3 Pt 1): 432-438, 1990

Human papillomavirus infection in male partners of females with condylomatous dysplasia of the cervix. Archivos Espanoles de Urologia 49(2): 112-116, 1996

Polymerase chain reaction for the diagnostic identification of HIV infection. Medicina 9(1): 41-43, 1989

Comparison of Southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human papillomavirus sequences in invasive carcinoma of the uterine cervix. Modern Pathology 5(2): 207-211, 1992

Detection of human papillomavirus DNA by molecular hybridization in tube Interest in cervical neoplasia. Annales De Biologie Clinique. 57(5): 589-593,.-., 1999

Comparison of ViraPap, Southern hybridization, and polymerase chain reaction methods for human papillomavirus identification in an epidemiological investigation of cervical cancer. Journal of Clinical Microbiology 30(11): 2951-2959, 1992

Detection of human papillomavirus DNA by AffiProbe HPV-DNA test kit in cervical scrapes or biopsies-histopathologic correlates. Infectious Diseases in Obstetrics and Gynecology 2(3): 126-129, 1994

Polymerase chain reaction identification of human immunodeficiency virus-1 in preserved human sclera. American Journal of Ophthalmology 118(4): 528-530, 1994

Prevalence of human papillomavirus types 11, 16 and 18 in cervical swabs. A study of 1362 pregnant women. European Journal of Obstetrics Gynecology and Reproductive Biology 35(2-3): 191-198, 1990

Comparison between sonodynamic effect with protoporphyrin IX and hematoporphyrin on sarcoma 180. Cancer Chemotherapy and Pharmacology 60(5): 671-680, 2000

Diagnostic sensitivity of different techniques in the diagnosis of lung tumors with the flexible fiberoptic bronchoscope comparison of brush biopsy imprint cytology of forceps biopsy and histology of forceps biopsy. Cancer 67(1): 72-75, 1991

Human papillomavirus DNA in fomites on objects used for the management of patients with genital human papillomavirus infections. Obstetrics and Gynecology 74(6): 950-954, 1989

A high frequency of hepatobiliary disease and suspected cholangiocarcinoma associated with heavy Opisthorchis viverrini infection in a small community in north-east Thailand. Transactions of the Royal Society of Tropical Medicine and Hygiene 84(5): 715-719, 1990