+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antigen presenting cell-independent cytokine and spontaneous in vitro IgE production in patients with atopic dermatitis: increased interferon-gamma production and lack of effects of in vivo low-dose interferon-gamma treatment

Antigen presenting cell-independent cytokine and spontaneous in vitro IgE production in patients with atopic dermatitis: increased interferon-gamma production and lack of effects of in vivo low-dose interferon-gamma treatment

Journal of Allergy and Clinical Immunology 96(1): 84-91

Atopic dermatitis is characterized by elevated serum IgE concentrations and dysregulation of T-lymphocyte function. To examine the pattern of cytokine production associated with elevated IgE levels, phorbol ester plus ionomycin-stimulated production of interleukin (IL)-4, IL-5, and interferon-gamma (IFN-gamma) by blood mononuclear cells from 16 patients with atopic dermatitis was compared with that of 18 healthy subjects. Spontaneous in vitro IgE production was also studied longitudinally in patients receiving placebo or daily treatment with 0.05 mg/m-2 IFN-gamma. Spontaneous in vitro IgE production and mitogen-driven IL-4 and IFN-gamma synthesis did not differ when patients were receiving interferon treatment compared with no treatment. Furthermore, ionomycin plus phorbol ester-stimulated mononuclear cells from patients with atopic dermatitis produced less IL-4 and more IFN-gamma than did cells from healthy subjects. IL-5 production by cells from patients with atopic dermatitis did not differ from that of cells from healthy subjects. The ratio of IL-4 to IFN-gamma produced in vitro was significantly lower (p = 0.04) in the cells of patients with atopic dermatitis (0.9) as compared with those of healthy subjects (2.7). The findings suggest that when circulating T cells are stimulated under antigen presenting cell-independent conditions, atopic dermatitis is not characterized by the shift in the reciprocal relationship between IL-4 and IFN-gamma production which has been postulated to explain the pathogenesis of IgE elevation and the therapeutic action of IFN-gamma in patients with atopic dermatitis.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 008172202

Download citation: RISBibTeXText

PMID: 7622767

Related references

Immunochemical characterization of recombinant and native tropomyosins as a new allergen from the house dust mite,. Journal of Allergy and Clinical Immunology 96(1): 74-83, 1995

Keratinocytes facilitate vascularization of acellular de epidermized dermis. Clinical Research 39(2): 515A, 1991

Interferon-gamma production specific for hepatitis B virus antigen by intrahepatic T lymphocytes in patients with acute and chronic hepatitis B. American Journal of Gastroenterology 89(1): 92-96, 1994

In vitro interferon-gamma release test in patients with drug-induced pemphigus. Israel Medical Association Journal 10(6): 424-427, 2008

The influence of interferon alpha and gamma, singly or in combination on human natural cell mediated cytotoxicity. Bioscience Reports 9(5): 549-557, 1989

Interferon-gamma production by T lymphocytes from renal cell carcinoma patients: evidence of impaired secretion in response to interleukin-12. Journal of ImmunoTherapy 22(1): 71-79, 1999

Diminished insulinotropic effects of gastrin-releasing peptide in pregnant sheep are reproduced by progesterone treatment of nonpregnant animals. Endocrinology 131(5): 2337-2343, 1992

Decreased integrin alpha-2, but normal response to TGF-beta in scleroderma fibroblasts. Journal of Dermatological Science 9(3): 169-175, 1995

Effects of exogenous hormones on the acetylcholine level of the middle hypothalamus in rat; effects of ACTH, DOCA and adrenocortical substances besides the adrenal cortex on the diencephalic acetylcholine level. II. Zentralblatt für Gynakologie 75(10): 373-376, 1953

Gamma interferon inhibits human luteal cell progesterone production in vitro. Journal of Interferon Research 10(Suppl. 1): S140, 1990

Interferon-gamma-inducing factor elicits antitumor immunity in association with interferon-gamma production. Journal of ImmunoTherapy 21(1): 48-55, 1998

Production and secretion of indole alkaloids in hairy root cultures of Catharanthus roseus: Effects of in situ adsorption, fungal elicitation and permeabilization. Journal of Fermentation and Bioengineering 78(3): 229-234, 1994

Effects of two novel bisphosphonates on bone cells in vitro. Bone and Mineral 26(2): 95-107, 1994

Interferon gamma ifn gamma decreases neutrophil accumulation at sites of acute inflammation. Clinical Research 37(2): 351A, 1989

CpG-oligodeoxynucleotides co-stimulate primary T cells in the absence of antigen-presenting cells. European Journal of Immunology 29(4): 1209-1218, 1999