+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Antimutagenicity of ellagic acid against aflatoxin B1 in the Salmonella microsuspension assay



Antimutagenicity of ellagic acid against aflatoxin B1 in the Salmonella microsuspension assay



Mutation Research 360(1): 15-21



Ellagic acid (EA) is a phenolic compound with antimutagenic and anticarcinogenic properties. It occurs naturally in some foods such as strawberries, raspberries, grapes, black currants and walnuts. In the present study, we used the Salmonella microsuspension assay to examine the antimutagenicity of EA against the potent mutagen aflatoxin B-1 (AFB-1) using tester strains TA98 and TA100. Further, we used a two-stage incubation procedure that incorporates washing the bacterial cells free of the incubation mixture after the first incubation to investigate EA and AFB-1 interaction. Three different concentrations of AFB-1 (2.5, 5 and 10 ng/tube) were tested against five different concentrations of EA for TA98 and TA100. EA significantly inhibited mutagenicity of all doses of AFB-1 in both tester strains with the addition of S9. EA alone was not mutagenic at the concentrations tested. The greatest inhibitory effect of EA on AFB-1 mutagenicity occurred when EA and AFB-1 were incubated together. Lower inhibition was apparent when the cells were first incubated with EA followed by a second incubation with AFB-1, and also when the cells were first incubated with AFB-1 followed by a second incubation with EA alone. The results of the sequential incubation studies support the hypothesis that one mechanism of inhibition could involve the formation of a chemical complex between EA and AFB-1.

(PDF emailed within 0-6 h: $19.90)

Accession: 008174210

Download citation: RISBibTeXText

PMID: 8657205

DOI: 10.1016/s0165-1161(96)90232-0


Related references

Inhibitory effects of ellagic acid on the direct-acting mutagenicity of aflatoxin B1 in the Salmonella microsuspension assay. Mutation Research 398(1-2): 183-187, 1998

Ellagic acid peracetate is superior to ellagic acid in the prevention of genotoxicity due to aflatoxin B1 in bone marrow and lung cells. Journal of Pharmacy and Pharmacology 59(1): 81-86, 2007

Inhibition of aflatoxin B 1 mutagenesis in Salmonella typhimurium and Dna damage in cultured rat and human tracheobronchial tissues by ellagic acid. Carcinogenesis 8(11): 1651-1656, 1987

Inhibition of aflatoxin B1 mutagenesis in Salmonella typhimurium and DNA damage in cultured rat and human tracheobronchial tissues by ellagic acid. Carcinogenesis 8(11): 1651-1656, 1987

Antimutagenicity testing of tannic acid and related compounds toward direct mutagens using modified Ames Salmonella/microsome assay. Abstracts of the General Meeting of the American Society for Microbiology 95(0): 515, 1995

Antimutagenicity of ellagic acid towards the food mutagen IQ: investigation into possible mechanisms of action. Food and Chemical Toxicology 30(4): 289-295, 1992

Miniaturization of the microsuspension Salmonella/microsome assay in agar microplates. Environmental and Molecular Mutagenesis 59(6): 488-501, 2018

Analysis of mutagens by the microsuspension mutagenicity assay in Salmonella typhimurium. Iarc Scientific Publications 1993(109): 300-306, 1993

Supercritical fluid chromatography coupled with the Salmonella microsuspension mutagenicity assay. Fresenius' Journal of Analytical Chemistry 344(10-11): 442-446, 1992

Genotoxicity of 5-aminolevulinic and 4,5-dioxovaleric acids in the salmonella/microsuspension mutagenicity assay and SOS chromotest. Environmental and Molecular Mutagenesis 40(1): 63-70, 2002

Comparative antimutagenicity of chlorophyllin and five other agents against aflatoxin B1-induced reversion in Salmonella typhimurium strain TA98. Teratogenesis, Carcinogenesis, and Mutagenesis 8(4): 215-224, 1988

Analysis of mutagens by the microsuspension forward-mutation assay in Salmonella typhimurium strain TM677. Iarc Scientific Publications 1993(109): 307-313, 1993

Microsuspension assay employing salmonella typhimurium yg strains for the evaluation of mutagenicity of indoor airborne particulates. Mutation Research 252(1): 108-109, 1991

Differences of mutagenic activity of airborne particulates by particle size: Assay by the Salmonella microsuspension procedure. Japanese Journal of Toxicology & Environmental Health 39(2): 139-147, 1993

Evaluation of antimutagenicity in the spiral salmonella assay. Environmental & Molecular Mutagenesis Supplement (20): 26, 1992