Apoptosis in the central nervous system of developing mouse fetuses from 5-azacytidine-administered dams
Hossain, M.M.; Nakayama, H.; Goto, N.
Toxicologic Pathology 23(3): 367-372
1995
ISSN/ISBN: 0192-6233 PMID: 7544907 DOI: 10.1177/019262339502300313
Accession: 008179653
To determine the effect of 5-azacytidine (5 Az), a cytidine analogue, on the developing fetus, different strains of mice were injected with various doses of the agent, ip, at 11 days of gestation. Light microscopically, severely pyknotic cell death profiles were observed in different layers of the developing brain and spinal cord (central nervous system) on the day after the injection. Electron microscopy of the pyknotic cells revealed the shrinkage of the cell body, to various degrees margination of the nuclear chromatin, condensation of nuclear materials, fragmentation of the affected cells, and, finally, neat "bite-size" bodies engulfed by surrounding normal neuroepithelial cells and macrophage-like cells. DNA fragmentation was detected in the nuclei of the pyknotic cells by an in situ detection method for fragmented DNA ends. These changes were consistent with the criteria of apoptosis.