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Application of the Western blotting method for the serological diagnosis of enterovirus infections. I. Detection of anti-coxsackie virus antibodies in diagnostic reference sera and in sera of experimentally infected mice



Application of the Western blotting method for the serological diagnosis of enterovirus infections. I. Detection of anti-coxsackie virus antibodies in diagnostic reference sera and in sera of experimentally infected mice



Epidemiologie Mikrobiologie Imunologie 46(2): 47-50



The introduction of rapid serological diagnosis of enterovirus infections is faced with the problem of inter-type cross-reactions. Therefore the method of choice is still the virus-neutralization test on cell cultures and on suckling mice resp., i.e. methods which are expensive and time consuming and demanding on laboratory equipment. The detection of antibodies against the Coxsackie virus by means of Western blotting revealed a specific reaction of commercial hyperimmune diagnostic rabbit sera as well as of sera obtained after oral infection of mice. The application of the method in the diagnosis of human enterovirus infections will be the subject of the subsequent part of this study.

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