+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle



Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle



Febs Letters 399(3): 307-309



Targeting of the cytoplasmic membrane protein leader peptidase (Lep) and a Lep mutant (Lep-inv) that inserts with an inverted topology compared to the wild-type protein was studied in Escherichia coli strains that are conditional for the expression of either Ffh or 4.5S RNA, the two components of the E. coli SRP. Depletion of either component strongly affected the insertion of both Lep and Lep-inv into the cytoplasmic membrane. This indicates that SRP is required for the assembly of cytoplasmic membrane proteins in E. coli.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008191168

Download citation: RISBibTeXText

PMID: 8985168

DOI: 10.1016/s0014-5793(96)01354-3


Related references

Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 95(25): 14646-14651, 1998

The functional integration of a polytopic membrane protein of Escherichia coli is dependent on the bacterial signal-recognition particle. European Journal of Biochemistry 233(3): 766-771, 1995

The integration of YidC into the cytoplasmic membrane of Escherichia coli requires the signal recognition particle, SecA and SecYEG. Journal of Biological Chemistry 277(8): 5715-5718, 2002

An amphiphilic region in the cytoplasmic domain of KdpD is recognized by the signal recognition particle and targeted to the Escherichia coli membrane. Molecular Microbiology 68(6): 1471-1484, 2008

Association of Escherichia coli ribosomes with the inner membrane requires the signal recognition particle receptor but is independent of the signal recognition particle. Proceedings of the National Academy of Sciences of the United States of America 97(9): 4621-4626, 2000

Signal recognition particle (SRP)-mediated targeting and Sec-dependent translocation of an extracellular Escherichia coli protein. Journal of Biological Chemistry 278(7): 4654-4659, 2003

Use of thioredoxin as a reporter to identify a subset of Escherichia coli signal sequences that promote signal recognition particle-dependent translocation. Journal of Bacteriology 187(9): 2983-2991, 2005

Small cytoplasmic RNA of Bacillus subtilis: functional relationship with human signal recognition particle 7S RNA and Escherichia coli 4.5S RNA. Journal of Bacteriology 174(7): 2185-2192, 1992

Wild type and mutant signal peptides of Escherichia coli outer membrane lipoprotein interact with equal efficiency with mammalian signal recognition particle. Journal of Biological Chemistry 262(20): 9463-9468, 1987

Conformational change of Escherichia coli signal recognition particle Ffh is affected by the functionality of signal peptides of ribose-binding protein. Molecules and Cells 27(6): 681-687, 2009

SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle. Journal of Biological Chemistry 274(13): 8993-8997, 1999

Signal recognition particle mediated protein targeting in Escherichia coli. Antonie van Leeuwenhoek 79(1): 17-31, 2001