+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Autocrine growth loop of the epidermal growth factor receptor in normal and immortalized human bronchial epithelial cells



Autocrine growth loop of the epidermal growth factor receptor in normal and immortalized human bronchial epithelial cells



Experimental Cell Research 223(2): 268-273



Epidermal growth factor (EGF) is a potent growth factor for human normal bronchial epithelial (HBE) cells and lung cancer cells, which often demonstrate an EGF receptor (EGFR) autocrine loop. We have found that HBE cells are capable of proliferating in basal medium without EGF supplementation, and this suggests the probable presence of an active EGFR autocrine loop in non-neoplastic HBE cells. Northern blot hybridization shows that the parental and immortalized HBE cells express comparable and high levels of mRNA for EGFR, transforming growth factor-alpha (TGF-alpha), and amphiregulin (AR), but not EGF. Incubation with neutralizing monoclonal antibodies (mAb) against EGFR partially inhibits the growth of these cells. Immunohistochemistry shows that HBE cells express the TGF-alpha peptide in vitro and in vivo, however, neutralizing mAbs against TGF-alpha fail to inhibit their proliferation. In contrast, AR stimulates the growth of HBE cells. Thus, several EGF-family ligands appear to be involved functionally in the EGFR autocrine growth loop in HBE cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008204392

Download citation: RISBibTeXText

PMID: 8601403

DOI: 10.1006/excr.1996.0081


Related references

Interferon gamma induces prostaglandin G/H synthase-2 through an autocrine loop via the epidermal growth factor receptor in human bronchial epithelial cells. Journal of Clinical Investigation 99(5): 1057-1063, 1997

The autocrine loop of epidermal growth factor receptor-epidermal growth factor / transforming growth factor-alpha in malignant rhabdoid tumor cell lines: heterogeneity of autocrine mechanism in TTC549. Japanese Journal of Cancer Research 92(3): 269-278, 2001

The Autocrine Loop of Epidermal Growth Factor Receptor-Epidermal Growth Factor/Transforming Growth Factor-α in Malignant Rhabdoid Tumor Cell Lines: Heterogeneity of Autocrine Mechanism in TTC549. Cancer Science 92(3): 269-278, 2001

A raf-independent epidermal growth factor receptor autocrine loop is necessary for Ras transformation of rat intestinal epithelial cells. Journal of Biological Chemistry 272(30): 18926-18931, 1997

Autocrine ligands for the epidermal growth factor receptor mediate interleukin-8 release from bronchial epithelial cells in response to cigarette smoke. American Journal of Respiratory Cell and Molecular Biology 27(1): 85-90, 2002

Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress. Cancer Research 65(18): 8242-8249, 2005

Altered gene expression of c myc epidermal growth factor receptor transforming growth factor alpha and c erb b2 in an immortalized human breast epithelial cell line hmt 3522 is associated with decreased growth factor requirements. Cancer Research 52(5): 1210-1217, 1992

Hepatocyte growth factor/scatter factor is an autocrine factor for human normal bronchial epithelial and lung carcinoma cells. Cell Growth & Differentiation 4(7): 571-579, 1993

Modulation by estrogen and growth factors of transforming growth factor-alpha and epidermal growth factor receptor expression in normal and malignant human mammary epithelial cells. Recent Results in Cancer Research. Fortschritte der Krebsforschung. Progres Dans les Recherches sur le Cancer 113: 57-69, 1989

Characterization of the transforming growth factor alpha epidermal growth factor receptor autocrine loop in human colon tumor cell lines. Proceedings of the American Association for Cancer Research Annual Meeting 31: 45, 1990

Hepatocyte growth factor/scatter factor is an autocrine factor expressed by human normal bronchial epithelial and non-small cell lung carcinoma cells. FASEB Journal 7(3-4): A429, 1993

Transforming growth factor alpha and amphiregulin as autocrine growth factors in nontransformed, immortalized 184A1N4 human mammary epithelial cells. Molecular & Cellular Differentiation 1(2): 163-184, 1993

Insulin-like growth factor binding protein secreted by the human prostate cancer cell line DU145 enhances binding of IGF-1 to its receptor and is regulated by the epidermal growth factor autocrine growth loop. Proceedings of the American Association for Cancer Research Annual Meeting 34: 237, 1993

Cytokine epithelial interaction induction of both components of an autocrine loop tgf alpha and epidermal growth factor receptor in pancreatic cancer cells by tnf alpha. Immunobiology 183(3-4): 263, 1991

Tumorigenicity of immortalized human prostate epithelial cells Association with decreased epidermal growth factor receptor expression. Proceedings of the American Association for Cancer Research Annual Meeting 35: 272, 1994