Section 9
Chapter 8,206

Automated high-performance liquid chromatographic method for the determination of nedocromil sodium in human urine using bimodal column switching

Baker, P.R.; Gardner, J.J.; Wilkinson, D.

Journal of Chromatography. B Biomedical Applications 668(1): 59-65


ISSN/ISBN: 1572-6495
PMID: 7550982
DOI: 10.1016/0378-4347(95)00049-o
Accession: 008205431

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An automated HPLC method is described for the determination of nedocromil sodium in human urine. An HPLC autosampler is used to inject urine samples onto a short reversed-phase column. This column acts as a concentration column and performs a preliminary extraction. The concentration column is automatically back-flushed onto an ion-exchange column where final separation of nedocromil sodium from urine constituents occurs. Recovery, accuracy, precision, sensitivity and specificity were investigated The method has been applied to urine samples from clinical studies, and the results were compared to those obtained using a radioimmunoassay developed previously.

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