+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Base analog N-6-hydroxylaminopurine mutagenesis in Escherichia coli: Genetic control and molecular specificity



Base analog N-6-hydroxylaminopurine mutagenesis in Escherichia coli: Genetic control and molecular specificity



Mutation Research 357(1-2): 1-15



We have studied the molecular specificity of the base analog N-6-hydroxylaminopurine (HAP) in the E. coli lacI gene, as well as the effects of mutations in DNA repair and replication genes on HAP mutagenesis. HAP induced base substitutions of the two transition types (A cntdot T fwdarw G cntdot C and G cntdot C fwdarw A cntdot T) at equal frequency. This bidirectional transition specificity is consistent with in vitro primer extension experiments with the Klenow fragment of DNA polymerase I in which we observed that either dTTP or dCTP were incorporated opposite HAP in an oligonucleotide template. The spectrum of HAP-induced transitions was different from the spontaneous transitions in either a wild-type or a mismatch-repair-defective (mutL) strain. Mutations in genes controlling excision repair, exonucleolytic proofreading, mismatch correction, error-prone (SOS) repair and 8-oxo-guanine repair did not affect HAP-induced mutagenesis substantially. However, an extensive deletion of several genes in the uvrB-bio region conferred supersensitivity to the lethal and mutagenic effects of HAP, perhaps due to an effect on HAP metabolism. dnaE antimutator alleles reduced HAP-forward mutagenicity in allele-specific manner: dnaE911 reduced it several fold, while dnaE915 abolished it almost completely. The results obtained are consistent with the idea that HAP is mutagenic in E. coli via a pathway generating replication errors.

(PDF emailed within 0-6 h: $19.90)

Accession: 008213642

Download citation: RISBibTeXText

PMID: 8876675

DOI: 10.1016/0027-5107(96)00060-7


Related references

Genetic control of metabolism of mutagenic purine base analogs 6-hydroxylaminopurine and 2-amino-6-hydroxylaminopurine in yeast Saccharomyces cerevisiae. Genetika 45(4): 471-477, 2009

Base analog 6-N-hydroxylaminopurine mutagenesis in the yeast Saccharomyces cerevisiae is controlled by replicative DNA polymerases. Mutation Research. 369(1-2): 33-44, 1996

The base analog 6-N-hydroxylaminopurine (HAP) mutagenesis is dependent on the integrity of the uvsE, uvsF and uvsB genes in Aspergillus nidulans. Antonie van Leeuwenhoek 71(3): 249-255, 1997

Mutagenic specificity of the base analog 6-N-hydroxylaminopurine in the URA3 gene of the yeast Saccharomyces cerevisiae. Mutagenesis 8(5): 417-421, 1993

Mutagenic specificity of the base analog 6-N-hydroxylaminopurine in the LYS2 gene of yeast Saccharomyces cerevisiae. Mutation Research 473(2): 151-161, 20 February, 2001

Effects of the umuDC, mucAB, and samAB operons on the mutational specificity of chemical mutagenesis in Escherichia coli: II. Base substitution mutagenesis. Mutation Research 314(1): 39-49, 1994

Multiple antimutagenesis mechanisms affect mutagenic activity and specificity of the base analog 6-N-hydroxylaminopurine in bacteria and yeast. Mutation Research. 402(1-2): 41-50, E 18, 1998

Overexpression of the yeast HAM1 gene prevents 6-N-hydroxylaminopurine mutagenesis in Escherichia coli. Acta Biochimica Polonica 45(3): 645-652, 1999

Effect of Escherichia coli dnaE antimutator mutants on mutagenesis by the base analog N4-aminocytidine. Mutation Research. 402(1-2): 23-28, E 18, 1998

Mutational specificity of the base analog 2 amino purine in escherichia coli. Mutation Research 83(1): 25-38, 1981

Hypersensitivity of Escherichia coli D(uvrB-bio) mutants to 6-hydroxylaminopurine and other base analogs is due to a defect in molybdenum cofactor biosynthesis. Journal of Bacteriology 182(12): 61-7, 2000

Genetic control of metabolism of a mutagenic analog of 6-N-hydroxylaminopurine bases in Saccharomyces cerevisiae yeasts. Genetika 33(5): 591-598, 1997

Hypersensitivity of Escherichia coli Delta(uvrB-bio) mutants to 6-hydroxylaminopurine and other base analogs is due to a defect in molybdenum cofactor biosynthesis. Journal of Bacteriology 182(12): 3361-3367, 2000

Genetic control of uv mutagenesis in escherichia coli. Journal of Radiation Research 19(1):, 1978

Umu c mediated mis repair mutagenesis in escherichia coli extent and specificity of sos mutagenesis. Mutation Research 111(1): 51-60, 1983