+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Binding of iron and inhibition of iron-dependent oxidative cell injury by the calcium chelator 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA)



Binding of iron and inhibition of iron-dependent oxidative cell injury by the calcium chelator 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA)



Biochemical Pharmacology 55(3): 287-295



A role for increases in intracellular calcium (Ca2+) has been suggested in the pathophysiology of various forms of oxidant-mediated cell injury. In recent studies, we found that iron bound to the Pseudomonas aeruginosa siderophore, pyochelin, augments oxidant-mediated endothelial cell injury by catalyzing the formation of hydroxyl radical (HO.). To investigate the role of Ca2+ in this process, the effects of two Ca2+ chelating agents, Fura-2 and 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA), were assessed. BAPTA, but not Fura-2, was protective against H2O2/ferripyochelin-mediated injury. Subsequent data suggested that chelation of iron rather than Ca2+ by BAPTA was most likely responsible. Spectrophotometry demonstrated that both ferrous (Fe2+) and ferric (Fe3+) iron formed a complex with BAPTA. The affinity of BAPTA for the metals was Fe3+ > Ca2+ > Fe2+. BAPTA was found to decrease markedly iron-catalyzed production of HO. and/or ferryl species when analyzed by spin trapping. Although our results do not definitively prove that BAPTA protects endothelial cells from ferripyochelin-associated damage by chelating iron, these data indicate that caution must be exercised in utilizing protective effects of intracellular "Ca2+ chelating agents" as evidence for a role of alterations in cellular Ca2+ levels in experimental conditions in which iron-mediated oxidant production is also occurring.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008223732

Download citation: RISBibTeXText

PMID: 9484794

DOI: 10.1016/s0006-2952(97)00463-2


Related references

Binding of ionic ligands to biopolymers. Biophysical Journal 70(2 Part 2): A135, 1996

Inhibitory effects of lipophilic acids and related compounds on bacteria and mammalian cells. Antimicrobial Agents and ChemoTherapy 7(3): 349-363, 1975

Inhibition of phosphoglucose isomerase. Nature 178(4547): 1401, 1956

1,2-dithiolan-3-ones and derivatives structurally related to leinamycin. Synthesis and biological evaluation. Farmaco 58(9): 995-998, 2003

Evidence for the interaction between antacid and gastric mucosa using an "artificial stomach" model including gastric mucosa. Journal of Pharmacology and Experimental Therapeutics 263(3): 1206-1211, 1992

Determination of 1, 4-dioxane in detergent chemicals by using SPME complex coating of phenylethylenethyl acrylate copolymer and PEG-20M. Se Pu 25(2): 286-287, 2007

Calcium-ion-induced stabilization of the protease from Bacillus cereus WQ9-2 in aqueous hydrophilic solvents: effect of calcium ion binding on the hydration shell and intramolecular interactions. Journal of Biological Inorganic Chemistry 18(2): 211-221, 2013

Carbachol inhibits Na(+)-K(+)-ATPase activity in choroid plexus via stimulation of the NO/cGMP pathway. American Journal of Physiology. Cell Physiology 279(6): C1685-C1693, 2000

Preparation of solutions for sulfanilamide preparation injections in pharmacy and in pharmaceutical manufacture. Farmatsiia 25(4): 24-28, 1976

Mechanism of complexation of the phenothiazine dye methylene blue with fullerene C60. Russian Journal of Physical Chemistry A, Focus on Chemistry 87(4): 662-667, 2013

ICL670A, a powerful new synthetic oral iron chelator Evaluation in hypertransfused rats and in cultured iron-loaded rat heart cells. Blood 96(11 Part 1): 225a, 2000

Effect of intraamniotic vitamin A on palatal closure of fetal rats. Indian Journal of Experimental Biology 38(5): 457-461, 2000

Inhibition of granuloma formation induced by potassium permanganate in the mouse by a specific human recombinant receptor antagonist for interleukin-1 (hrIL-1ra). Cellular Immunology 147(2): 446-457, 1993

SIGNe: A Geographic Information System on the Web for End-Stage Renal Disease. Studies in Health Technology and Informatics 116: 713-718, 2005

Iron Balance and the Role of Hepcidin in Chronic Kidney Disease. Seminars in Nephrology 36(2): 87-93, 2016