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Biosynthesis of glucoamylase from Aspergillus niger by solid-state fermentation using tea waste as the basis of a solid substrate



Biosynthesis of glucoamylase from Aspergillus niger by solid-state fermentation using tea waste as the basis of a solid substrate



Bioresource Technology. 65(1-2): 83-85, Y-.



Tea waste, supplemented with minerals, was used as the solid substrate for the synthesis of glucoamylase by a strain of Aspergillus niger NCIM 1248. Maximum glucoamylase synthesis (198.4 IU/g dry substrate (gds)) was obtained on the mineralized tea waste at 96 h with an inoculum, initial moisture and pH of 4%, 60% and 4.5, respectively at 30degree C Among different carbon sources, sucrose favoured the increase of glucoamylase synthesis (212.6 IU/gds) in 96 h, whereas among different nitrogen sources, malt extract gave the highest enzyme titre (226 0 IU/gds) at 72 h.

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Accession: 008233452

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DOI: 10.1016/s0960-8524(98)00012-1



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