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Ca2+ binding sites of the ryanodine receptor/Ca2+ release channel of sarcoplasmic reticulum. Low affinity binding site(s) as probed by terbium fluorescence



Ca2+ binding sites of the ryanodine receptor/Ca2+ release channel of sarcoplasmic reticulum. Low affinity binding site(s) as probed by terbium fluorescence



Journal of Biological Chemistry 269(40): 24864-9



Fluorescence spectroscopy has been used to study the interaction of Tb-3+ (as a Ca-2+ analog) with the purified ryanodine receptor (RyR)/Ca-2+ release channel of skeletal muscle sarcoplasmic reticulum. Tb-3+ replaces Ca-2+ in both the high- and the low-affinity sites. Occupation of the low-affinity site (inhibitory), but not of the high-affinity Ca-2+ binding site (activating), by Tb-3+ results in a strong enhanced green fluorescence (at 543 nm) and in an inhibition of ryanodine binding.

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Accession: 008253648

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PMID: 7929166


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