Callus formation from protoplast cultures of Allium wakegi

Seo, B.B.; Lee, S.H.; Nair, A.S.

Bionature 14(1): 23-28


Accession: 008258250

Download citation:  

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Protoplasts were enzymatically isolated from leaves obtained from bulb cultures of amphidiploid A. wakegi, and protoplasts were inoculated in KM8P liquid medium containing 0.1M Mannitol, 0.4m glucose. 0.4 mg/1 2,4-D, 2 mg/1 NAA and 1 mg/1 BAP, and incubated in dark. Colony formation was observed within two weeks of inoculation, and developed into mini-callus after one month. The mini-callus obtained were transferred to MS medium containing 3% sucrose, 0.4 mg/1 2,4-D, 2 mg/1 NAA. 1 mg/1 BAP, and 0.8% agarose, and developed into friable callus.