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Catecholamine secretion, calcium levels and calcium influx in response to membrane depolarization in bovine chromaffin cells



Catecholamine secretion, calcium levels and calcium influx in response to membrane depolarization in bovine chromaffin cells



Neuroscience. 68(1): 265-272



In this paper, we show that exposure of chromaffin cells to high K+ (75 mM) for 5 min releases about 15% of total norepinephrine and 8% of total epinephrine contained in chromaffin cells. The measured resting membrane potential of these cells was - 55 mV. Long (10 s) depolarizing electrical pulses applied from a holding potential of - 55 mV to 5 mV. that would produce a depolarization similar to exposure to high K+ (75 mM), induced an inward Ca-2+ current that inactivated with a time constant of about 0.8 s and promoted the influx of about 1 fmol of Ca-2+ into the cell. Both high K+ and electrically-induced depolarization increased intracellular Ca-2+ levels to a similar value (about 350 nM). Extrapolation would indicate that total Ca-2+ influx in high K+ (75 mM)-stimulated 10-6 chromaffin cells would amount to 1 nmol which would promote the secretion of about 4.9 nmol of norepinephrine and 3.5 nmol of epinephrine from 10-6 chromaffin cells. The results indicate that Ca-2+ influx in response to depolarization is short-lived, likely due to Ca-2+-dependent inactivation of voltage-dependent Ca-2+ channels. However, intracellular Ca-2+ levels remain high as long as depolarization is present and long after Ca-2+ influx has ceased. This would suggest that some processes related to either Ca-2+ buffering or extrusion from the cell may be voltage dependent.

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Accession: 008270607

Download citation: RISBibTeXText

PMID: 7477931

DOI: 10.1016/0306-4522(95)00132-3


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