+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Characterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase



Characterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase



Journal of Bacteriology 177(17): 5148-5150



Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, revealed one protein species of 82,000 Da. This protein was found even in cells in which the amyE gene, encoding exocellular alpha-amylase, was disrupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.

(PDF emailed within 0-6 h: $19.90)

Accession: 008294642

Download citation: RISBibTeXText

PMID: 7665495

DOI: 10.1128/jb.177.17.5148-5150.1995


Related references

Characterization of a new cell-bound a-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular a-amylase. Journal of Bacteriology 177(17): 48-50, 1995

Genetic and biochemical studies on cell-bound alpha-amylase in Bacillus subtilis Marburg. Journal of Bacteriology 119(2): 425-430, 1974

Molecular cloning, nucleotide sequencing, and expression of the Bacillus subtilis (natto) IAM1212 alpha-amylase gene, which encodes an alpha-amylase structurally similar to but enzymatically distinct from that of B. subtilis 2633. Journal of Bacteriology 172(9): 4901-4908, 1990

Studies on the primary structure of .ALPHA.-amylase from Bacillus subtilis var. amylosacchariticus. Part IV. Alignments of tryptic peptides in CNBr fragments of .ALPHA.-amylase from Bacillus subtilis var. amylosacchariticus. Agricultural and Biological Chemistry 49(7): 1933-1942, 1985

Enhancement of alpha amylase production by integrating and amplifying the alpha amylase gene of bacillus amyloliquefaciens in the genome of bacillus subtilis. Applied Microbiology & Biotechnology 27(1): 64-71, 1987

Studies on the primary structure of .ALPHA.-amylase from Bacillus subtilis var. amylosacchariticus. Part III. Isolation and alignment of CNBr fragments of .ALPHA.-amylase from Bacillus subtilis var. amylosacchariticus. Agricultural and Biological Chemistry 49(7): 1923-1931, 1985

Tunicamycin accumulates cell bound alpha amylase and protease in bacillus subtilis and bacillus amyloliquefaciens. Abstracts of the Annual Meeting of the American Society for Microbiology (79): 208, 1979

Genes affecting the productivity of alpha-amylase in Bacillus subtilis Marburg. Journal of Bacteriology 121(2): 688-694, 1975

Complete nucleotide sequence of an alpha-amylase gene from Bacillus subtilis 2633, an alpha-amylase extrahyperproducing strain. Nucleic Acids Research 16(14b): 7178, 1988

N-terminal amino acid sequence of .ALPHA.-amylase from Bacillus subtilis var. amylosacchariticus: Comparison with that of "liquefying" type .ALPHA.-amylase. Agricultural and Biological Chemistry 44(1): 215-216, 1980

Molecular cloning and expression of an alpha amylase gene from an alpha amylase extrahyper producing bacillus subtilis. Agricultural & Biological Chemistry 52(2): 399-406, 1988

Molecular cloning and expression of an .ALPHA.-amylase gene from an .ALPHA.-amylase extrahyper producing Bacillus subtilis. Agricultural and Biological Chemistry 52(2): 399-406, 1988

Alpha-amylase genes (amyR2 and amyE+) from an alpha-amylase-hyperproducing Bacillus subtilis strain: molecular cloning and nucleotide sequences. Journal of Bacteriology 156(1): 327-337, 1983

Accumulation of cell-bound alpha-amylase in Bacillus subtilis cells in the presence of tunicamycin. Biochemical and Biophysical Research Communications 92(1): 334-341, 1980

On the nature of enz alpha amylase forming system in bacillus subtilis stability of the messenger rna for enz alpha amylase. Journal of Fermentation Technology 46(6): 427-436, 1968