+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of two cDNAs for novel drought-inducible genes in the highly drought-tolerant cowpea



Characterization of two cDNAs for novel drought-inducible genes in the highly drought-tolerant cowpea



Journal of Plant Research 109(1096): 415-424



Ten cDNAs for drought-inducible genes were isolated using differential screening of a cDNA library prepared from 10-hr dehydrated cowpea plants, Vigna unguiculata (S. Iuchi, K Yamaguchi-Shinozaki, T. Urao, T. Terao, K. Shinozaki; Plant Cell Physiology, 1996 in press). Two of the cDNA clones, designated CPRD12 and CPRD46, were sequenced and characterized. The CPRD12 and CPRD46 cDNAs encode putative proteins related to nonmetallo short-chain alcohol dehydrogenase (CPRD12) and chloroplastic lipoxygenase (CPRD46). Northern blot analysis revealed that these genes are induced by high-salinity stress and exogenous abscisic acid, but not by cold stress. The CPRD46 gene is also responsive to heat stress and methyl jasmonate and salicylic acid. Genomic Southern blot analysis suggested that CPRD12 constitutes a small gene family, but that CPRD46 is a single copy gene. We discuss the possible functions of these two CPRD gene products under drought stress.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008303507

Download citation: RISBibTeXText

DOI: 10.1007/bf02344557


Related references

Novel drought-inducible genes in the highly drought-tolerant cowpea: cloning of cDNAs and analysis of the expression of the corresponding genes. Plant and Cell Physiology 37(8): 1073-1082, 1996

VuDREB2A, a novel DREB2-type transcription factor in the drought-tolerant legume cowpea, mediates DRE-dependent expression of stress-responsive genes and confers enhanced drought resistance in transgenic Arabidopsis. Planta 240(3): 645-664, 2014

Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. Journal of Experimental Botany 60(12): 3531-3544, 2009

Differential Expression of Two-Component SystemRelated Drought-Responsive Genes in Two Contrasting Drought-Tolerant Soybean Cultivars DT51 and MTD720 Under Well-Watered and Drought Conditions. Plant Molecular Biology Reporter 33(5): 1599-1610, 2015

Identification of drought-responsive genes in a drought-tolerant cotton (Gossypium hirsutumL.) cultivar under reduced irrigation field conditions and development of candidate gene markers for drought tolerance. Molecular Breeding 34(4): 1777-1796, 2014

Effect of drought stress on the yield and composition of volatile oils of drought-tolerant and non-drought-tolerant clones of Tagetes minuta. Planta Medica 68(5): 472-474, 2002

A drought- and abscisic acid - inducible H1 histone gene from a drought-tolerant tomato, Lycopersicon pennellii Effects of over-expression in transgenic tobacco. Journal of Cellular Biochemistry Suppl 0(19A): 159, 1995

Differential response to drought and abscisic acid of two cDNAs corresponding to genes expressed during drought conditioning in jack pine seedlings. New forests 13(1-3): 165-176, 1997

Seedling stage drought-induced phenotypes and drought-responsive genes in diverse cowpea genotypes. Crop Science 48(2): 541-552, 2008

Isolation of cowpea genes conferring drought tolerance: Construction of a cDNA drought expression library. Water SA 33(3): 387-391, 2007

Identification of two new genes from drought tolerant peanut up-regulated in response to drought. Plant growth regulation 52(3): 249-258, 2007

A stress-inducible gene for 9-cis-epoxycarotenoid dioxygenase involved in abscisic acid biosynthesis under water stress in drought-tolerant cowpea. Plant Physiology 123(2): 553-562, 2000

Analysis of drought tolerant and susceptible maize genotypes using SSR markers tagging candidate genes and consensus QTLs for drought tolerance. Indian Journal of Genetics and Plant Breeding 69(4): 344-351, 2009

Drought - inducible genes and differentially expressed sequence tags associated with components of drought tolerance in durum wheat. 2008

Metabolic responses to drought stress in the tissues of drought-tolerant and drought-sensitive wheat genotype seedlings. Aob Plants 1(2): ply 016, 2018