+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Chemiluminescence ELISA for the detection of antibodies to bovine leukaemia virus antigens



Chemiluminescence ELISA for the detection of antibodies to bovine leukaemia virus antigens



Journal of Bioluminescence and Chemiluminescence 10(3): 193-198



A chemiluminescence enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine leukaemia virus antigens (BLV) has been developed. The possibility of using an enhanced chemiluminescence reaction for the determination of adsorbed immunoperoxidase conjugates was studied in this work. The intensity of chemiluminescence depends on both the concentration of reagents and experimental conditions used. The efficiency of the assay is determined by the formation of an immobilized antigen monolayer. A relationship between the quantity of the protein added and adsorbed has been shown. The optimal time and temperature for the antigen-antibody incubation steps have been estimated for each system (3 h at 37 degrees C was chosen as a standard incubation time). A linear dependence of the chemiluminescence intensity and optical density on the concentration of antibodies to the BLV antigens was observed. The detection limit of antibodies in the chemiluminescence ELISA is 2-3 times lower than that in the spectrophotometric one. The results obtained indicate the possibility of using both methods.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008306569

Download citation: RISBibTeXText

PMID: 7676862

DOI: 10.1002/bio.1170100308


Related references

Use of two monoclonal antibodies in an ELISA test for the detection of antibodies to bovine leukaemia virus envelope protein gp51. Journal of Virological Methods 23(2): 211-222, 1989

Use of monoclonal antibody in an ELISA test for the detection of antibodies to bovine leukaemia virus. Journal of Virological Methods 6(1): 19-29, 1983

Evaluation of an indirect ELISA for the detection of antibodies to bovine leukaemia virus in milk and serum. Journal of virological methods 33(3): 319-333, 1991

Comparative evaluation of the spectrophotometric and chemiluminescent detection of antibodies against bovine leukemia virus antigens in elisa. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii (7): 64-66, 1991

BoLA antigens are associated with increased frequency of persistent lymphocytosis in bovine leukaemia virus infected cattle and with increased incidence of antibodies to bovine leukaemia virus. Animal Genetics 19(1): 151-158, 1988

Development and evaluation of a Luminex multiplex serology assay to detect antibodies to bovine herpes virus 1, parainfluenza 3 virus, bovine viral diarrhoea virus, and bovine respiratory syncytial virus, with comparison to existing ELISA detection methods. Journal of Immunological Methods 366(1-2): 79-88, 2011

Chicken antibodies: a useful tool for antigen capture ELISA to detect bovine leukaemia virus without cross-reaction with other mammalian antibodies. Veterinary Research Communications 31(1): 43-51, 2007

Use of monoclonal antibodies in an ELISA for the diagnosis of bovine leukaemia virus infection. Journal of virological methods 30(1): 79-87, 1990

Detection of antibodies to bovine leukemia virus in bovine milk samples with an ELISA involving two monoclonal antibodies. Veterinary Microbiology 18(1): 89-93, 1988

Infectivity of bovine leukaemia virus infected cattle: an ELISA for detecting antigens expressed in in vitro cultured lymphocytes. Veterinary Microbiology 30(2-3): 137-150, 1992

Epidemiological evaluation of a monoclonal ELISA detecting antibodies against bovine leukaemia virus in serum pools. Epidemiology and Infection 113(3): 563-569, 1994

Development of an experimental ELISA for the detection of antigens and antibodies to the virus of Pacheco disease using monoclonal antibodies. Journal of Veterinary Medicine Series B 41(6): 369-380, 1994

Indirect method ELISA for the detection of antibodies against bovine diarrhea virus in bovine serum. Revista Argentina de Microbiologia 35(1): 19-23, 2003

The use of monoclonal antibodies in elisa for detection of antibodies to bovine viral diarrhea virus. Journal of Veterinary Medicine Series B 34(5): 356-363, 1987

Rodent and bovine filarial antigens in detection of antibodies in human bancroftian filariasis by ELISA. Indian Journal of Medical Research 73(Suppl.): 93-96, 1981