+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cholera toxin B subunit (CTB) entrapped in microparticles shows comparable immunogenicity to CTB mixed with whole cholera toxin following oral immunization

Cholera toxin B subunit (CTB) entrapped in microparticles shows comparable immunogenicity to CTB mixed with whole cholera toxin following oral immunization

International Journal of Pharmaceutics (Amsterdam) 119(2): 251-255

Cholera toxin B subunit (CTB) was entrapped in poly(lactide-co-glycolide) microparticles and the responses induced by microencapsulated CTB were comparable to those induced by oral immunization with CTB mixed with whole cholera toxin. In addition, the CTB was released from microparticles intact following in vitro incubation, as determined by the ability of the CTB to bind to its cellular receptor, GM1. Moreover, microencapsulated CTB induced serum antibodies against intact CTB and not against monomeric fragments.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 008311497

Download citation: RISBibTeXText

DOI: 10.1016/0378-5173(95)00046-l

Related references

Oral immunization of dogs with purified cholera toxin, crude cholera toxin, or B subunit: evidence for synergistic protection by antitoxic and antibacterial mechanisms. Infection and Immunity 37(2): 687-694, 1982

The adjuvant effect of cholera toxin cholera toxin b subunit and glutaraldehyde modified cholera toxin on the mucosal immune response of rabbits to 2 acetylaminofluorene thyroglobulin conjugates. FASEB Journal 4(3): A500, 1990

Immunogenicity of two formulations of oral cholera vaccine comprised of killed whole vibrios and the B subunit of cholera toxin. Infection and Immunity 57(1): 117-120, 1989

Synergistic action of cholera toxin B subunit (and Escherichia coli heat-labile toxin B subunit) and a trace amount of cholera whole toxin as an adjuvant for nasal influenza vaccine. Vaccine 12(5): 419-426, 1994

Protection against experimental cholera in the rat. A study on the formation of antibodies against cholera toxin and desensitization of adenylate cyclase after immunization with cholera toxin. International Archives of Allergy and Applied Immunology 75(2): 143-148, 1984

Protective Oral Cholera Vaccine Based on a Combination of Cholera Toxin B Subunit and Inactivated Cholera Vibrios. Wachsmuth, I K, Blake, P A, Olsvik, O Vibrio cholerae and cholera: Molecular to global perspectives: 415-424, 1994

Protection of rats against cholera toxin and cholera-like enterotoxins by immunization with enteric-coated cholera toxin. Annals of Tropical Paediatrics 6(3): 199-204, 1986

Mucosal immunization with pneumococcal polysaccharide-cholera toxin B subunit conjugate entrapped in alginate microsphere. Abstracts of the General Meeting of the American Society for Microbiology 97: 256, 1997

Cautionary note on the use of the B subunit of cholera toxin as a ganglioside GM1 probe: detection of cholera toxin A subunit in B subunit preparations by a sensitive adenylate cyclase assay. Journal of Cellular Biochemistry 42(3): 143-152, 1990

Cholera toxin zonula occludens toxin and accessory cholera enterotoxin gene-negative Vibrio cholerae non-01 strains produce the new cholera toxin. Current Science (Bangalore) 70(12): 1041-1043, 1996

Priming immunization against cholera toxin and E. coli heat-labile toxin by a cholera toxin short peptide-beta-galactosidase hybrid synthesized in E. coli. Embo Journal 4(12): 3339-3343, 1985

Immunization with cholera toxin B subunit induces high-level protection in the suckling mouse model of cholera. Plos one 8(2): E57269, 2013

Clone and express ctb-stx2b fusion gene in Enterohemrrhagic escherichia coli O157:H7 Shigeal toxin 2B subunit and V cholera toxin B subunit and the detection of their immunogenicity. Zhonghua Liu Xing Bing Xue Za Zhi 29(4): 378-382, 2008

Reduction in oral immunogenicity of cholera toxin B subunit by N-terminal peptide addition. Infection and Immunity 61(2): 384-390, 1993

Interneuronal transfer of axonally transported proteins: studies with HRP and HRP conjugates of wheat germ agglutinin, cholera toxin and the B subunit of cholera toxin. Brain Research 311(2): 366-369, 1984