Chymotrypsin inhibitors in potato tubers infected with Phytophthora infestans

Valueva, T.A.; Kladnitskaya, G.V.; Il'inskaya, L.I.; Gerasimova, N.G.; Ozeretskovskaya, O.L.; Mosolov, V.V.

Bioorganicheskaya Khimiya 24(5): 346-349


ISSN/ISBN: 0360-4497
Accession: 008317971

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Chymotrypsin inhibitor proteins that accumulate in diffusates of potato tubers infected with Phytophthora infestans zoospores were studied. The inhibitors were isolated by affinity chromatography on chymotrypsin-Sepharose. It was shown that the proteins inhibit the growth of hyphae and the germination of zoospores of the phytophthora fungus. The N-terminal sequence analysis of the inhibitors that predominated in diffusates of infected tubers suggests that the 22-kDa protein and the 21-kDa protein (which consists of two polypeptide chains with molecular masses of 16.5 and 4.5 kDa) that are present in healthy tubers can accumulate in potato tubers infected with the fungus.