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Citrate buffer alternative to picric acid for Masson Trichrome stain



Citrate buffer alternative to picric acid for Masson Trichrome stain



Journal of Histotechnology 19(4): 341-342



An alternative to picric acid as a mordant in the staining of cytoplasm, keratin, muscle fibers, and intercellular fibers in the Masson trichrome stain is a citrate acid-sodium citrate buffer used as the microwave pretreatment of the tissue mounted on a slide. The Masson trichrome stain, a combination of Biebrich scarlet-acid fuchsin and aniline blue dye can be done within 1 hr and avoids the hazards associated with picric acid, a highly explosive compound in the dry state.

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Accession: 008320629

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DOI: 10.1179/his.1996.19.4.341


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