+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Clinical evaluation of various selected ELISA kits for the detection of anti-DNA antibodies



Clinical evaluation of various selected ELISA kits for the detection of anti-DNA antibodies



Lupus 4(5): 370-374



Fifty four coded sera, 38 from eight patients with systemic lupus erythematosus (SLE), four from one patient with systemic vasculitis, one from one patient with polyarthritis and 11 normal controls were tested for anti-dsDNA antibodies using seven commercial enzyme linked immunosorbent assays (ELISA) and the radioimmunoassay method (RIA) routinely used in our unit. Sensitivity, specificity and predictive values were tested for both SLE diagnosis and disease activity. Using anti-dsDNA antibodies as a diagnostic test for SLE there were differences in sensitivity (from 66% to 95%), specificity (from 75% to 100%), predictive positive values (from 89% to 100%) and predictive negative values (from 50% to 87%) among ELISA kits. The RIA method was either more specific or equal to ELISA kits. Using 'equivocal' values as positive values an increase in sensitivity was observed but at the expense of specificity. Similar differences and trends were observed when the results were used as a measure for disease activity. This suggests that there are differences in sensitivity, specificity and predictive values among ELISA kits both in the diagnosis of SLE as well as in the determination of disease activity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008325993

Download citation: RISBibTeXText

PMID: 8563731

DOI: 10.1177/096120339500400507


Related references

Evaluation of eight ELISA kits for the detection of anti-LAV/HTLV-III antibodies. Lancet 1(8490): 1152-1153, 1986

Comparative study of ELISA and indirect immunofluorescence for the detection of anti-neutrophil cytoplasmic antibodies. Evaluation of the SCIMEDX/EURO Diagnostica ELISA assay in a clinical setting. American Journal of Clinical Pathology 104(6): 667-672, 1995

Evaluation of five commercial assays for the detection of anti-dsDNA antibodies: three Crithidia luciliae indirect immunofluorescence test kits and two enzyme immunoassay kits. Journal of the Medical Association of Thailand 97(2): 220-224, 2014

Evaluation of commercial ELISA kits for the detection of antibodies against bluetongue virus. Polish Journal of Veterinary Sciences 14(4): 615-619, 2011

Evaluation of two ELISA kits for the detection of Aujeszky's disease antibodies in pigs. Veterinary Record 131(17): 391-393, 1992

Circulating immune complex levels measured by new ELISA kits utilizing monoclonal anti-C1q and anti-C3d antibodies correlate with clinical activities of SLE but not with those of RA. Journal of Clinical and Laboratory Immunology 38(4): 161-173, 1992

Clinical evaluation of a modified ELISA, using photobiotinylated DNA, for the detection of anti-DNA antibodies. Journal Of Immunological Methods. 170(1): 93-102, 1994

Evaluation of two commercial ELISA test kits for the detection of pseudorabies antibodies in pigs. Journal of Veterinary Diagnostic Investigation 2(4): 350-352, 1990

Evaluation of various ELISA kits for the detection of antibodies to M. avium subsp. paratuberculosis in cattle. Tierarztliche Umschau 56(12): 648-654, 2001

Evaluation of three commercial ELISA test kits for the detection of antibodies against Salmonella enteritidis. Tierarztliche Praxis 22(4): 350-357, 1994

ELISA kits for detection of anti-HCV antibodies. "Viral Hepatitis" Working Group of the French Society of Blood Transfusion. Transfusion Clinique et Biologique 1(1): 47-48, 1994

Evaluation of the specificity of two commercial ELISA kits for the detection of swollen head syndrome (Pneumovirus) antibodies. Proceedings of Western Poultry Disease Conference: 5th) 355-358, 1996

Evaluation of commercial ELISA test kits for the detection of antibodies against the virus of Gumboro disease in vaccinated flock. Arquivos do Instituto Biologico Sao Paulo 70(1): 55-59, 2003

Evaluation of three commercial ELISA kits for anticardiolipin and anti-beta2-glycoprotein I antibodies in the laboratory diagnosis of the antiphospholipid syndrome. International Journal of Laboratory Hematology 33(1): 97, 2011

Development, validation, and application of ELISA for detection of anti-HD105 antibodies in pre-clinical safety evaluation using monkeys. Journal of Pharmaceutical and Biomedical Analysis 131: 309-315, 2016