+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Cloning and expression in Escherichia coli of the gene encoding an extracellular deoxyribonuclease (DNase) from Aeromonas hydrophila

Cloning and expression in Escherichia coli of the gene encoding an extracellular deoxyribonuclease (DNase) from Aeromonas hydrophila

Gene (Amsterdam) 122(1): 175-180

The gene encoding an extracellular DNase from Aeromonas hydrophila CHC-1 has been cloned and sequenced. Following expression of the dns in Escherichia coli, it was revealed that some of the cloned enzyme was present in the cell-free extracellular supernatant fluid, and there was no cell lysis and concurrent release of cytoplasmic or periplasmic proteins. Therefore, results suggest that E. coli cells were capable of secreting the DNase extracellularly, albeit very inefficiently. The dns is transcribed from its own promoter in E. coli, and expressed as a 25-kDa product, as determined by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the culture supernatant preparations followed by a DNA-hydrolysis assay. Nucleotide sequence analysis predicted a single open reading frame of 690 bp encoding a 230-amino acid (aa) polypeptide, with a potential 20-aa signal peptide located at the N terminus of the predicted protein. The deduced aa sequence of the entire protein is highly homologous with that of the DNase of Vibrio cholerae.

(PDF emailed within 0-6 h: $19.90)

Accession: 008332295

Download citation: RISBibTeXText

PMID: 1452026

DOI: 10.1016/0378-1119(92)90046-R

Related references

Cloning and expression of a chitinase gene from Aeromonas hydrophila in Escherichia coli. Applied and Environmental Microbiology 57(8): 2426-2428, 1991

Cloning and expression of an aeromonas hydrophila chitinase gene in escherichia coli. Current Microbiology 21(5): 329-337, 1990

Cloning, expression, and mapping of the Aeromonas hydrophila aerolysin gene determinant in Escherichia coli K-12. Journal of Bacteriology 167(1): 368-374, 1986

Molecular cloning and expression in Escherichia coli of the structural gene for the hemolytic toxin aerolysin from Aeromonas hydrophila. Molecular & General Genetics 204(2): 289-295, 1986

Cloning and sequence analysis of the gene (eprA1) encoding an extracellular protease from Aeromonas hydrophila. Gene (Amsterdam) 199(1-2): 225-229, 1997

Cloning, sequencing, and characterization of the nucH gene encoding an extracellular nuclease from Aeromonas hydrophila JMP636. Journal of Bacteriology 178(13): 3926-3933, 1996

The gene encoding a periplasmic deoxyribonuclease from Aeromonas hydrophila. FEMS Microbiology Letters 173(1): 41-46, April 1, 1999

Extracellular proteins of Vibrio cholerae: molecular cloning, nucleotide sequence and characterization of the deoxyribonuclease (DNase) together with its periplasmic localization in Escherichia coli K-12. Gene 53(1): 31-40, 1987

Cloning, expression and characterization of aerolysin from Aeromonas hydrophila in Escherichia coli. Indian Journal of Biochemistry & Biophysics 44(4): 204-208, 2007

Cloning, expression in Escherichia coli, and enzymatic properties of laccase from Aeromonas hydrophila WL-11. Journal of Environmental Sciences 22(4): 635-640, 2010

Molecular cloning of polyhydroxyalkanoate synthesis operon from Aeromonas hydrophila and its expression in Escherichia coli. Biotechnology Progress 20(5): 1332-1336, 2004

Molecular cloning and heterologous expression of laccase from Aeromonas hydrophila NIU01 in Escherichia coli with parameters optimization in production. Applied Biochemistry and Biotechnology 169(7): 2223-2235, 2013

Cloning and expression of a gene encoding an immunogenic extracellular protein of haemophilus influenzae type b in escherichia coli. Abstracts of the Annual Meeting of the American Society for Microbiology 86: 90, 1986

Purification, gene cloning, amino acid sequence analysis, and expression of an extracellular lipase from an Aeromonas hydrophila human isolate. Applied and Environmental Microbiology 59(8): 2411-2417, 1993

Cloning and expression in Escherichia coli of a synthetic gene encoding the extracellular domain of the human muscle acetylcholine receptor alpha-subunit. Gene 98(2): 289-293, 1991