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Cloning, sequencing, and expression of a Thermomonospora fusca protease gene in Streptomyces lividans

Cloning, sequencing, and expression of a Thermomonospora fusca protease gene in Streptomyces lividans

Applied and Environmental Microbiology 62(11): 4256-4259

The major Thermomonospora fusca YX extracellular protease gene (tfpA) was cloned into Escherichia coli and Streptomyces lividans and was sequenced. The open reading frame encoded 375 residues, including a 31-residue potential signal sequence, an N-terminal prosequence containing 150 residues, and the 194-residue mature protease that belongs to the chymotrypsin family. The protease was secreted by S. lividans, but evidence suggested that it was bound to an extracellular protease inhibitor. An inhibitor-deficient mutant was selected to produce protease for purification.

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Accession: 008336264

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PMID: 8900021

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