Column-switching solid-phase trace-enrichment high-performance liquid chromatographic method for measurement of buprenorphine and norbuprenorphine in human plasma and urine by electrochemical detection

Schleyer, E.; Lohmann, R.; Rolf, C.; Gralow, A.; Kaufmann, C.C.; Unterhalt, M.; Hiddemann, W.

Journal of Chromatography 614(2): 275-283


ISSN/ISBN: 0021-9673
PMID: 8314940
DOI: 10.1016/0378-4347(93)80319-y
Accession: 008343769

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We describe a new high-performance liquid chromatographic method using electrochemical detection for the determination of buprenorphine and norbuprenorphine in plasma and urine. The minimum concentration for detection of buprenorphine and norbuprenorphine is 40 pg/ml. The intra-assay coefficient of variation (C.V.) in plasma and urine samples ranges from 6 to 17% depending on the drug concentration. At a plasma concentration of 500 pg/ml the inter-assay C.V. is 8% for buprenorphine and 9% for norbuprenorphine. The analysis duration is 16 min. After solid-phase extraction and evaporation a valve-switching system with two Rheodyne valves enables sample enrichment, optimal sample cleaning and rapid elution of long-retained substances.