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Combined bromodeoxyuridine immunohistochemistry and masson trichrome staining: Facilitated detection cell proliferation in viable vs. infarcted myocardium



Combined bromodeoxyuridine immunohistochemistry and masson trichrome staining: Facilitated detection cell proliferation in viable vs. infarcted myocardium



Biotechnic and Histochemistry 67(5): 253-255



Cells in the S-phase of the cell cycle can be identified as tissue sections by immunohistochemical localization of the thymidine analogue bromodeoxyuridine (BrdU). Generally, a single counterstain is used to visualize the underlying tissue; however, interpretation of morphologic detail is often difficult. We have utilized BrdU to localize proliferating cells in myocardium exposed to angiogenic mitogens. To facilitate identification of labelled nuclei in the context of infracted vs. viable myocardium, BrdU immunohistochemistry was followed by a modified Masson trichrome stain. The time of exposure to the counterstains and the wash protocol were rerevised, permitting clear identification of the labelled brown nuclei against a background of red viable myocardium vs. blue infarct. The combined technique also provides color contrast suitable for computer-based image analysis.

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Accession: 008344649

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PMID: 1284404


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