Comparative study of solid-phase extraction cartridges in the simultaneous reversed-phase HPLC analysis of bamifylline and its major metabolite AC-119 in biological fluids

Papadoyannis, I.; Samanidou, V.; Zotou, A.; Tsioni, G.

Journal of Liquid Chromatography 16(17): 3827-3845


ISSN/ISBN: 0148-3919
DOI: 10.1080/10826079308019670
Accession: 008352690

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Several different solid-phase extraction cartridges provided by different manufacturers were investigated for the optimization of bamifylline and its major metabolic AC-119 isolation from samples of biological interest: human blood plasma and urine. The drug and its metabolite were subsequently analysed by HPLC after separation on a RP-18 Lichrosorb column 250 times 4.6 mm, 10 mu-m, with caffeine as internal standard. Total analysis time was approximately 10 min, while the sample volume required was low, 40 mu-l for blood plasma and 100 mu-l for urine samples. Data with respect to recovery, precision and accuracy and limits of detection are reported and discussed.