+ Translate

Comparison of effects of uroguanylin, guanylin, and Escherichia coli heat-stable enterotoxin STa in mouse intestine and kidney: Evidence that uroguanylin is an intestinal natriuretic hormone

, : Comparison of effects of uroguanylin, guanylin, and Escherichia coli heat-stable enterotoxin STa in mouse intestine and kidney: Evidence that uroguanylin is an intestinal natriuretic hormone. Journal of Investigative Medicine 45(5): 276-283

Background: Uroguanylin and guanylin are intestinal peptides that activate a receptor-guanylate cyclase, which is also a receptor for Escherichia coli heat-stable enterotoxin (STa). These peptides may have a role in the body's regulation of fluid and electrolytes. Methods: STa, bioactive guanylin, and bioactive uroguanylin were evaluated for effects in: 1) the suckling mouse intestinal fluid secretion assay; 2) an in vitro suckling mouse intestinal loop assay; 3) an intestinal receptor autoradiography assay; 4) a control or agonist-stimulated assay for cGMP response in T84 cells; and 5) an in vivo renal function assay in mice. Results: In vivo, orally administered uroguanylin and STa but not guanylin, stimulated intestinal fluid secretion. All three peptides activated intestinal guanylate cyclase and had common intestinal receptors. In vitro, after pretreatment with chymotrypsin, only uroguanylin and STa retained agonist activity. Chymostatin preserved guanylin activity. STa and uroguanylin induced diuresis, natriuresis, and kaliuresis. Guanylin was less potent than uroguanylin and STa. Conclusions: The results suggest that the endogenous intestinal peptides, uroguanylin and guanylin, regulate water and electrolyte homeostasis both through local effects on intestinal epithelia and endocrine effects on the kidney.

(PDF 0-2 workdays service)

Order PDF Full Text


Not the article you are looking for?
Click here to order the paper you need

Accession: 008357393

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Marten, J., 1975: New cattle barns tested in practice. Landtechnik 30 (3) 104-110

Morris, RKA.; Parsons, MS., 1992: A survey of invertebrate communities on the shingle of Dungeness, Rye Harbour and Orford Ness. JNCC Report, i-iii, 1-241 No. 77

Kordulska, Franciszka, 1959: The treatment of gastric and duodenal ulcers with mucoprotein preparations. Robuden was given both by deep intramuscular injection and also 3 times a day before meals per os to 38 patients. Of these one 46-year-old woman went into a coma after the 7th injection and died 2 days later. All other patients responded favorably...

Anonymous, 2011: Mechanical and thermal characteristics of amaranth starch isolated by acid wet-milling procedure. Effects of soaking conditions during acid wet- milling of amaranth grain on mechanical and thermal properties of amaranth starch were investigated. A factorial design based on temperature (4-6 C) and SO2concentration (.1-1. g/L) was used. Dynamic...

Appalanarasiah, P., 1961: Review of work on Sugarcane smut. The author (Sugarcane Res. Sta., Anakapalle, Andhra Pradesh) deals with the symptoms, the taxonomy of Ustilago scitaminea, mode of infection, physiology, control, var. susceptibility, breeding for resistance, and economic losses.

Norris, Ken; Asase, Alex; Collen, Ben; Gockowksi, Jim; Mason, John; Phalan, Ben; Wade, Amy, 2010: Biodiversity in a forest-agriculture mosaic - the changing face of West African rainforests. The biodiversity of West African rainforests is globally significant but poorly described, little understood in terms of its functional significance, and under threat from forest loss and degradation. Estimates suggest that about 10 million ha of...

Balducci, Lodovico; Extermann, Martine, 2000: Management of the frail person with advanced cancer. Critical Reviews in Oncology-Hematology 33(2): 143-148, Feb

Tsai, S. S.; Sun, A. Y.; Kim, H. D.; Sun, G. Y., 1993: Manganese exposure to PC-12 cells alters triacylglycerol metabolism and promotes neurite outgrowth. Exposure of PC-12 cells to MnCl-2 (10-100 mu-M for 1 to 3 days) resulted in both morphological and metabolic changes as indicated by promotion of neurite outgrowth and an increased incorporation of labeled fatty acids (14:0, 18:1 and 20:4) into tr...

Glooschenko W.A.; Glooschenko V.A., 1969: Thermal pollution rivers estuaries steam generating power plant cooling water discharges plants animals. Florida Naturalist 42(1): 3-8

Scull B. J.; Felix C. J.; McCaleb S. B.; Shaw W. G., 1966: The inter-discipline approach to paleoenvironmental interpretations. Transactions - Gulf Coast Association of Geological Societies 16: 81-117