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Comparison of haemolytic activity between Fusobacterium necrophorum subsp. necrophorum and Fusobacterium necrophorum subsp. funduliforme in vitro and in vivo

Shinjo, T.; Misawa, N.; Goto, Y.

Apmis: Acta Pathologica Microbiologica et Immunologica Scandinavica 104(1): 75-78

1996


ISSN/ISBN: 0903-4641
PMID: 8645462
Accession: 008358161

Haemolytic activity of two subspecies of Fusobacterium necrophorum was compared in vitro and in vivo. F. necrophorum subsp. necrophorum (Fnn) showed a stronger activity than F. necrophorum subsp. funduliforme (Fnf) in vitro. Haemolytic activity of Fnn and Fnf was 57.97%+-1.90 and 17.33%+-1.44, respectively, compared to complete haemolysis by distilled water. In the mice injected with Fnn, haemolysin was detected in the liver at a titre of from 1:16 to 1:L28, and Fnn was recovered from all mice at a viable bacterial count of 10-5 to 10-6 cells per gram liver tissue. In the mice injected with Fnf, haemolysin titre was lt 1:2 to 1:32. No liver abscess was formed. The viable count of recovered bacteria was 10-3 to 10-5 cells per gram, except for two mice in which no Fnf was detected. The results suggest that haemolysin might be a virulence factor in this species.

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