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Comparison of three commercial enzyme-linked immunosorbent assays and virus neutralization test for the detection of antibodies to infectious bursal disease



Comparison of three commercial enzyme-linked immunosorbent assays and virus neutralization test for the detection of antibodies to infectious bursal disease



Journal of the Chinese Society of Veterinary Science 21(3): 152-159



Sera were collected, aliquot, and stored at -20C until tested by three commercial enzyme-linked immunosorbent assays (ELISA) and virus neutralization (VN) test. Good correlation among ELISA kits was indicated by the results, with the highest between Flockchek and Proflok ELISA titers (R value = 0.919). The R values of Trop ELISA to Flokchek and Trop ELISA to Proflok ELISA were 0.787 and 0.735, respectively. Good correlations among ELISA titers and VN titers were also found. Trop ELISA titers had the highest coefficient of correlation (R = 0.835) with VN titers, while Flockchek ELISA titers (R value = 0.720) and Proflok ELISA titers (R value = 0.672) also correlated well to VN titers. However, high variation existed in ELISA titers corresponding to each of specific VN titers. Highest coefficient of variation in Proflok ELISA titers was 141%, in Flockchek ELISA titers 164%, and in Trop ELISA titers 182%. Since high variations existed in ELISA titers to specific VN titers, collecting suitable amounts of serum samples for IBD antibody profile monitoring is advised.

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