Construction of intermediate vector expressing antisense RNA gene for the assembly origin of TMV-RNA
Peng, H.; Shen, X.R.; Wu, J.H.; Gong, Z.X.
Virologica Sinica 7(4): 426-431
ISSN/ISBN: 1674-0769 Accession: 008379638
In this paper we have designed and constructed an antisense gene which is complementary with the assembly origin of TMV-RNA, than a intermediate vector which contains the expressing unit, consisting of CaMV 35S promoter, antisense RNA gene and poly A signal, as well as the NPT-II expressing unit was constructed. The NPT-II is essential for the selection and transformation of plants Thus the successful construction of this intermediate vector will facilitate obtaining the transgenic plants.