EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Contribution of individual residues to formation of the native-like tertiary topology in the alpha-lactalbumin molten globule


Contribution of individual residues to formation of the native-like tertiary topology in the alpha-lactalbumin molten globule



Journal of Molecular Biology 280(1): 167-174



ISSN/ISBN: 0022-2836

PMID: 9653039

DOI: 10.1006/jmbi.1998.1826

Molten globules are partially folded forms of proteins that have native-like secondary structure and a compact geometry, but often without rigid, specific side-chain packing. Recently, the molten globule of alpha-lactalbumin (alpha-LA) has been shown to adopt a native-like tertiary topology, mainly localized in the alpha-helical domain. This native-like topology is reflected by the high effective concentration (Ceff) for formation of the 28-111 disulfide bond, which is approximately 10 to 40 times higher than the Ceff for formation of any non-native disulfide bond in the alpha-helical domain. In order to understand the mechanism for formation of the native-like tertiary topology, we substituted alanine for each of the 23 buried residues in the alpha-helical domain of alpha-LA and determined the effect of these substitutions on the Ceff for formation of the 28-111 disulfide bond. Our results indicate that a subset of hydrophobic residues is most important for formation of the native-like topology. These residues form a densely packed core in the three-dimensional structure of alpha-LA. In contrast, the less important residues consist of both hydrophobic and hydrophilic amino acids located at peripheral positions. These results suggest that a relatively small number of hydrophobic residues may be sufficient for specifying the overall structure of a protein during early stages of protein folding.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008383583

Download citation: RISBibTeXText

Related references

Contribution of individual residues to formation of the native-like tertiary topology in the a-lactalbumin molten globule. Journal of Molecular Biology 280(1): 7-74, 1998

Determinants of the native-like tertiary topology in the alpha-lactalbumin molten globule. Kuwajima, Kunihiro Author, Arai, Munehito Author International Congress Series; Old and new views of protein folding 145-154, 1999

The native-like tertiary fold in molten globule alpha-lactalbumin appears to be controlled by a continuous phase transition. Journal of Molecular Biology 261(3): 341-347, 1996

Contribution of the 6-120 disulfide bond of alpha-lactalbumin to the stabilities of its native and molten globule states. Biochemistry 31(50): 12695-12700, 1992

Vibrational Raman optical activity of alpha-lactalbumin: comparison with lysozyme, and evidence for native tertiary folds in molten globule states. Journal of Molecular Biology 254(4): 747-760, 1995

The native-like tertiary fold in molten globule a-lactalbumin appears to be controlled by a continuous phase transition. Journal of Molecular Biology 261: 1-7, 1996

Contribution of the 6-120 disulfide bond of α-lactalbumin to the stabilities of its native and molten globule states. Biochemistry (Easton) 31(50): 12695-12700, 1992

Contribution of the 6-120 disulfide bond of a-lactalbumin to the stabilities of its native and molten globule states. Biochemistry (American Chemical Society) 31: 695-700, 1992

Direct measurement of the energetics of the molten globule and native states of alpha lactalbumin. Biophysical Journal 61(2 Part 2): A477, 1992

Action of protein-glutaminase on alpha-lactalbumin in the native and molten globule states. Journal of Agricultural and Food Chemistry 49(12): 5999-6005, 2001

Non two-state denaturing transition from native to molten globule states of apo-alpha-lactalbumin. Biophysical Journal 72(2 Part 2): A394, 1997

Comparison of intramolecular packings in human alpha -lactalbumin in native and molten globule states. Studia Biophysica 112(2/3): 207-211, 1986

Rotational diffusion studies of native and molten globule states of bovine alpha lactalbumin. Studia Biophysica 126(2): 87-97, 1988

Vibrational Raman optical activity of a-lactalbumin: comparison with lysozyme, and evidence for native tertiary folds in molten globule states. Journal of Molecular Biology 254: 7-60, 1995

Different subdomains are most protected from hydrogen exchange in the molten globule and native states of human alpha-lactalbumin. Journal of Molecular Biology 253(5): 651-657, 1995