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Conversion of furostanol glycoside to spirostanol glycoside by beta-glucosidase in Costus speciosus

Conversion of furostanol glycoside to spirostanol glycoside by beta-glucosidase in Costus speciosus

Phytochemistry 41(3): 725-727

ISSN/ISBN: 0031-9422

A diosgenin-rich plant, Costus speciosus (Koenig) Smith was found to contain beta-glucosidase which converts a furostanol glycoside, protogracillin, to a spirostanol glycoside, gracillin. It was demonstrated that the enzyme was present in rhizomes of plants grown in a greenhouse and whole parts of plantlets cultured in vitro. The soluble enzyme from the cultivated rhizomes showed pH optima of 5.0 and the apparent Km for protogracillin of 0.10 mM. Activity of the enzyme was inhibited by typical beta-glucosidase inhibitors, such as D-glucono-1,5-lactone and conduritol-beta-epoxide.

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Accession: 008388085

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