+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coronavirus pseudoparticles formed with recombinant M and E proteins induce alpha interferon synthesis by leukocytes

Coronavirus pseudoparticles formed with recombinant M and E proteins induce alpha interferon synthesis by leukocytes

Journal of Virology 72(11): 8636-8643

Transmissible gastroenteritis virus (TGEV), an enteric coronavirus of swine, is a potent inducer of alpha interferon (IFN-alpha) both in vivo and in vitro. Incubation of peripheral blood mononuclear cells with noninfectious viral material such as inactivated virions or fixed, infected cells leads to early and strong IFN-alpha synthesis. Previous studies have shown that antibodies against the virus membrane glycoprotein M blocked the IFN induction and that two viruses with a mutated protein exhibited a decreased interferogenic activity, thus arguing for a direct involvement of M protein in this phenomenon. In this study, the IFN-alpha-inducing activity of recombinant M protein expressed in the absence or presence of other TGEV structural proteins was examined. Fixed cells coexpressing M together with at least the minor structural protein E were found to induce IFN-alpha almost as efficiently as TGEV-infected cells. Pseudoparticles resembling authentic virions were released in the culture medium of cells coexpressing M and E proteins. The interferogenic activity of purified pseudoparticles was shown to be comparable to that of TGEV virions, thus establishing that neither ribonucleoprotein nor spikes are required for IFN induction. The replacement of the externally exposed, N-terminal domain of M with that of bovine coronavirus (BCV) led to the production of chimeric particles with no major change in interferogenicity, although the structures of the TGEV and BCV ectodomains markedly differ. Moreover, BCV pseudoparticles also exhibited interferogenic activity. Together these observations suggest that the ability of coronavirus particles to induce IFN-alpha is more likely to involve a specific, multimeric structure than a definite sequence motif.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 008391187

Download citation: RISBibTeXText

PMID: 9765403

Related references

Interferon alpha inducing property of coronavirus particles and pseudoparticles. Advances in Experimental Medicine and Biology 440: 377-386, 1998

Coronavirus transmissible gastroenteritis virus-mediated induction of IFN alpha-mRNA in porcine leukocytes requires prior synthesis of soluble proteins. Veterinary Research 25(1): 29-36, 1994

Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: a study with pig leukocytes. Research in Immunology 144(2): 111-120, 1993

Recombinant human interferon-alpha and interferon-beta interleukin-6 but not interferon-gamma induce megakaryocytic differentiation of the acute megakaryoblastic leukaemia blast cells. Pathology Research & Practice 189(6-7): 712, 1993

Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus. Virology 380(1): 99, 2008

Decreased interferon synthesis and responsiveness to interferon by leukocytes from multiple sclerosis patients given natural alpha interferon. Journal of Interferon Research 4(3): 423-432, 1984

The induction of interferon-alpha and interferon-beta mRNA in human natural interferon-producing blood leukocytes requires de novo protein synthesis. Journal of Interferon Research 11(6): 371-377, 1991

Reconstituted coronavirus TGEV virosomes lose the virus ability to induce porcine interferon-alpha production. Veterinary Research 28(2): 105-114, 1997

Human leucocyte interferon-alpha therapy can induce a second response in treatment of thrombocytosis in patients with neutralising antibodies to recombinant interferon-alfa 2a. European Journal of Cancer 30a(11): 1729-1730, 1994

Induction of proteins in interferon-alpha- and interferon-gamma-treated polymorphonuclear leukocytes. Journal of Leukocyte Biology 45(5): 396-400, 1989

Influence of recombinant interferon alpha rifn alpha recombinant interferon gamma rfn gamma and recombinant tumor necrosis factor alpha rtnf alpha on hematopoietic precursor cells from healthy individuals and patients with cml comparison of in vitro sensitivity with the in vitro response to rifn alpha. Immunobiology 175(1-2): 62, 1987

In vitro sensitivity of hematopoietic precursor cells to recombinant interferon alpha recombinant interferon gamma and recombinant tumor necrosis factor alpha in normal controls and in patients with cml relationship to the in vivo response. Najman, A , Et Al (Ed ) Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 162 Les Inhibiteurs De L'hematopoiese; (Inserm (National Institute Of Health And Medical Research) Colloquium, Vol 162 The Inhibitors Of Hematopoiesis); First International Symposium on Inhibitory Factors in The Regulation Of Hematopoiesis, Paris, France, April 26-28, Xix+356p John Libbey Eurotext Ltd : Montrouge, France; Editions Inserm: Paris, France Illus Paper 325-3, 1987

Unmethylated CPG-containing oligodeoxynucleotides induce high levels of interferon-alpha in porcine leukocytes. Journal of Interferon & Cytokine Research 22(Suppl. 1): S-172, 2002

Properties of human alpha-interferon F and hybrid interferon F/D obtained from recombinant bacteria compared to the properties of an interferon preparation from human leukocytes. Doklady Akademii Nauk SSSR 268(4): 996, 1983

Interferon-alpha and interferon-beta induce Ia expression in limited protein synthesis condition. Journal of Interferon Research 10(5): 487-496, 1990