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Crystallization and preliminary X-ray study of the cathepsin B complexed with CA074, a selective inhibitor



Crystallization and preliminary X-ray study of the cathepsin B complexed with CA074, a selective inhibitor



Journal of Molecular Biology 227(3): 942-944



Cathepsin B from bovine spleen has been purified and crystallized as a complex with a specific inhibitor CA074 (N-(L-3-trans-propylcarbamoyloxirane-2-carbonyl)-L-isoleucyl-L-proline), using the hanging-drop-method. The complex crystals obtained from 50 mM-citrate buffer (pH 3.5) belonging to the tetragonal space group P4-1 (or P4-3) with a = 73 cntdot 06 ANG and c = 141 cntdot 59 ANG and the diffract beyond 2 cntdot 2 ANG resolution. There are two complex molecules per asymmetric unit giving a packing density of 3 cntdot 37 ANG -3/Da and indicating high solvent content of 63 cntdot 5%.

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Accession: 008402672

Download citation: RISBibTeXText

PMID: 1404397

DOI: 10.1016/0022-2836(92)90234-b


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