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Crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY



Crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY



Acta Crystallographica Section D Biological Crystallography 54(1): 140-142,. 1



The histidine-containing phosphotransfer (HPt) domain at the C-terminus of the anaerobic sensor kinase ArcB has been cocrystallized with the chemotaxis response regulator CheY by a hanging-drop vapor-diffusion method. Crystals belong to space group P21212, with unit-cell dimensions a = 55.32, b = 76.29 and c = 83.89 ANG, with one molecule in the crystallographic asymmetric unit. The crystals defrocked to 2.7 ANG resolution. This is the first crystallization of a protein-protein complex formed by a transmitter domain of sensor kinase and a receiver domain of response regulator in the two-component signal-transduction system.

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Accession: 008402848

Download citation: RISBibTeXText

PMID: 9761838

DOI: 10.1107/s0907444997007075


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