+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Cytopathology of liver and kidney in rainbow trout Oncorhynchus mykiss after long-term exposure to sublethal concentrations of linuron



Cytopathology of liver and kidney in rainbow trout Oncorhynchus mykiss after long-term exposure to sublethal concentrations of linuron



Diseases of Aquatic Organisms 21(1): 35-52



Hepatic and renal cytopathological alterations in fingerling rainbow trout Oncorhynchus mykiss following 5 wk exposure to 30, 120, and 240 mu-g l-1 linuron (3(3,4-dichlorophenyl)-1-methoxy-1-methylurea) were studied by electron microscopy. Ultrastructural alterations were detected in liver and kidney at gtoreq 30 mu-g l-1, 2 orders of magnitude below conventional LC-0. The response suggested a dose-response relationship with a change from adaptive to degenerative features at 120 mu-g l-1. Hepatocyte changes included: stimulation of mitosis; segmentation of nuclei; partial reorganization of heterochromatin; multiplication of nucleoli; fractionation, vesiculation and transformation of rough endoplasmic reticulum (RER) into myelinated bodies; induction of smooth endoplasmic reticulum; moderate steatosis; apparent proliferation of mitochondria, peroxisomes, Golgi fields and lysosomal elements; depletion of glycogen; perisinusoidal lipid accumulation; elevated rate of hepatocytes in various stages of necrosis; infiltration and increased phagocytic activity of macrophages. Reactions of renal tubular cells were differentiated in different nephron segments. Major alterations by site in kidney were (1) renal corpuscle: lobulation of podocyte nuclei; (2) proximal segment I: elevated heterogeneity of all cell components, increased heterochromatin and nuclear size, rearrangement of RER, proliferation of Golgi fields, novel lysosomal elements, decreased mitochondria and lysosomes at 240 mu-g l-1; (3) proximal segment II: nuclear lobulation, binucleated cells, proliferation of lysosomes and peroxisomes (lower concentrations), decreased peroxisomes and mitochondria (240 mu-g l-1), crystalline inclusions in lysosomal matrix, fragmentation, degranulation and circular arrangement of RER; (4) distal segment: induction of giant mitochondria with longitudinal crystalline inclusions, atypical lysosomes with long crystalline matrix inclusions, and augmentation of various lysosomal elements. Comparison of linuron-induced cellular alterations with cytopathological effects by potential linuron breakdown products, namely 4-chloraniline and 3,4-dichloroaniline, revealed a high degree of similarity of cytopathological phenomena, indicating that part of the changes observed after linuron exposure might well be due to the action of linuron metabolites.

(PDF emailed within 1 workday: $29.90)

Accession: 008412800

Download citation: RISBibTeXText


Related references

Histomorphometric and biochemical studies on the liver of rainbow trout (Oncorhynchus mykiss) after exposure to sublethal concentrations of phenol. Toxicology and Industrial Health 29(9): 856-861, 2014

Branchial morphological and endocrine responses of rainbow trout (Oncorhynchus mykiss) to a long-term sublethal acid exposure in which acclimation did not occur. Canadian Journal of Fisheries and Aquatic Sciences 50(1): 198-209, 1993

The effects of acute waterborne exposure to sublethal concentrations of molybdenum on the stress response in rainbow trout, Oncorhynchus mykiss. Plos One 10(1): E0115334, 2015

Segment specificity of the cytological response in rainbow trout (Oncorhynchus mykiss) renal tubules following prolonged exposure to sublethal concentrations of atrazine. Ecotoxicology and Environmental Safety 32(1): 39-50, 1995

Sublethal effects of prolonged exposure to disulfoton in rainbow trout (Oncorhynchus mykiss): cytological alterations in the liver by a potent acetylcholine esterase inhibitor. Ecotoxicology and Environmental Safety 34(1): 43-55, 1996

Transchem project - Part II: Transgenerational effects of long-term exposure to pendimethalin at environmental concentrations on the early development and viral pathogen susceptibility of rainbow trout (Oncorhynchus mykiss). Aquatic Toxicology 202: 126-135, 2018

Effects of sublethal concentrations of triphenyltinacetate on rainbow trout (Oncorhynchus mykiss). Archives of Environmental Contamination and Toxicology 30(3): 327-334, 1996

Chronic exposure of rainbow trout ( Oncorhynchus mykiss ) to simulated climate warming and sublethal ammonia: a year-long study of their appetite, growth, and metabolism. Canadian Journal of Fisheries and Aquatic Sciences 55(3): 576-586, 1998

The effects of chronic acetaminophen exposure on the kidney, gill and liver in rainbow trout (Oncorhynchus mykiss). Aquatic Toxicology 198: 20-29, 2018

Cytological alterations in the liver of rainbow trout Oncorhynchus mykiss after prolonged exposure to low concentrations of waterborne endosulfan. Diseases of Aquatic Organisms 25(1-2): 39-52, 1996

Short term and long term effects of ammonium chloride on rna dna and protein concentrations in muscle and serum in the rainbow trout oncorhynchus mykiss. Comparative Biochemistry & Physiology B 99(4): 899-902, 1991

Histopathological changes in rainbow trout (Oncorhynchus mykiss) after exposure to sublethal composite nitrogen fertilizers. Ecotoxicology and Environmental Safety 72(7): 1999-2004, 2009

Effects of sublethal ammonia exposure on swimming performance in rainbow trout (Oncorhynchus mykiss). Journal of Experimental Biology 204(Pt 15): 2691-2698, 2001

Effect of sublethal concentrations of four chemicals on susceptibility of juvenile rainbow trout (Oncorhynchus mykiss) to saprolegniosis. Applied and Environmental Microbiology 57(6): 1813-1816, 1991

Effects of copper at sublethal concentrations on growth and biochemical parameters in rainbow trout Oncorhynchus mykiss. International Review of Hydrobiology 98(2): 71-79, 2013