+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cytotoxic T-cell-resistant variants arise at early times after infection in C57BL/6 but not in SCID mice infected with a neurotrophic coronavirus



Cytotoxic T-cell-resistant variants arise at early times after infection in C57BL/6 but not in SCID mice infected with a neurotrophic coronavirus



Journal of Virology 71(10): 7640-7647



Under certain conditions, C57BL/6 mice persistently infected with mouse hepatitis virus strain JHM (MHV-JHM) develop clinical disease and histological evidence of demyelination several weeks after inoculation with virus. In a previous report, we showed that mutations in the RNA encoding an immunodominant CD8 T-cell epitope within the surface glycoprotein (epitope S-510-518) were present in all persistently infected animals and that these mutations abrogated recognition by virus-specific cytotoxic T cells (CTLs) in direct ex vivo cytotoxicity assays. To obtain further evidence that these mutations were necessary for the development of clinical disease, the temporal course of their appearance was determined. Mutations in the epitope were identified by 10 to 12 days after inoculation, and in some mice, virus containing mutated epitope was the dominant species detected by 15 days. In addition, most mice that remain asymptomatic at 80 days after inoculation, a time after which clinical disease almost never develops, were infected with only wild-type virus. Finally, analysis of virus isolated from mice with severe combined immunodeficiency (SCID) revealed the presence only of wild-type epitope S-510-518. These results, by showing that mutations are not selected in SCID mice and occur at early times after inoculation in C57BL/6 mice, support the view that they result from immune pressure and contribute to virus persistence and demyelination in mice infected persistently with MHV-JHM.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008413728

Download citation: RISBibTeXText

PMID: 9311846


Related references

Cytotoxic lymphocyte activity in the cns of theilers virus infected resistant c57bl 10 mice. Neurology 40(4 Suppl. 1): 219, 1990

Attenuated nephritis in inducible nitric oxide synthase knockout C57BL/6 mice and pulmonary hemorrhage in CB17 SCID and recombination activating gene 1 knockout C57BL/6 mice infected with Leptospira interrogans. Infection and Immunity 79(7): 2936-2940, 2011

Infection with cytotoxic T-lymphocyte escape mutants results in increased mortality and growth retardation in mice infected with a neurotropic coronavirus. Journal of Virology 72(7): 5912-5918, 1998

INOS-producing inflammatory dendritic cells constitute the major infected cell type during the chronic Leishmania major infection phase of C57BL/6 resistant mice. Plos Pathogens 5(6): E1000494, 2009

Effects of sex and age on the susceptibility of C57BL/6J mice to infection with Brachylaima cribbi and the course of infection in NOD SCID mice. Parasitology Research 88(7): 668-674, 2002

Demonstration of a splenic cytotoxic effector cell in mice of genotype scid/scid.bg/bg. Cellular Immunology 130(1): 106-117, 1990

Differences in the kinetics of T cell accumulations in C3H/HeN (Bcg-resistant) and C57BL/6 (Bcg-susceptible) mice infected with Mycobacterium paratuberculosis. Comparative Immunology Microbiology and Infectious Diseases 19(4): 289-304, 1996

Role of natural killer cells on engraftment of human lymphoid cells and on metastasis of human T-lymphoblastoid leukemia cells in C57BL/6J-scid mice and in C57BL/6J-scid bg mice. Cellular Immunology 171(2): 186-199, 1996

Antiviral activity of NK 1.1+ natural killer cells in C57BL/6 scid mice infected with murine cytomegalovirus. Natural Immunity 13(5): 239-245, 1994

Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c. International Journal of Molecular Medicine 37(3): 716-726, 2016

The cytotoxic T-cell response to herpes simplex virus type 1 infection of C57BL/6 mice is almost entirely directed against a single immunodominant determinant. Journal of Virology 73(9): 7619-7626, 1999

T cell-independent recovery from early acute cryptosporidiosis in Cryptosporidium parvum-infected neonatal SCID mice. Gastroenterology. 122(4 Suppl. 1): A-144, Il, 2002

Human Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes home preferentially to and induce selective regressions of autologous EBV-induced B cell lymphoproliferations in xenografted C.B-17 scid/scid mice. Journal of Experimental Medicine 183(3): 1215-1228, 1996

Human Epstein-Barr virus -specific cytotoxic T lymphocytes home preferentially to and induce selective regressions of autologous B-cell EBV-induced lymphoproliferations in xenografted CB-17 SCID/SCID mice. Blood 86(10 Suppl. 1): 462A, 1995

BLT-humanized C57BL/6 Rag2-/-γc-/-CD47-/- mice are resistant to GVHD and develop B- and T-cell immunity to HIV infection. Blood 122(25): 4013-4020, 2013