DNA fingerprinting by random amplified polymorphic DNA and restriction fragment length polymorphism is useful for yeast typing

Paffetti, D.; Barberio, C.; Casalone, E.; Cavalieri, D.; Fani, R.; Fia, G.; Mori, E.; Polsinelli, M.

Research in Microbiology 146(7): 587-594

1995


ISSN/ISBN: 0923-2508
PMID: 8578000
DOI: 10.1016/0923-2508(96)80565-1
Accession: 008416136

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Abstract
Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent with those derived from restriction fragment length polymorphism (RFLP) using two S. cerevisiae DNA probes. We conclude that RAPD fingerprinting, combined with the analysis of RFLP, can provide unambiguous type assignment in yeasts.