Section 9
Chapter 8,424

Deficient differentiation of mast cells in the skin of mi/mi mice. Usefulness of in situ hybridization for evaluation of mast cell phenotype

Kasugai, T.; Oguri, K.; Jippo-Kanemoto, T.; Morimoto, M.; Yamatodani, A.; Yoshida, K.; Ebi, Y.; Isozaki, K.; Tei, H.; Tsujimura, T.

American Journal of Pathology 143(5): 1337-1347


ISSN/ISBN: 0002-9440
PMID: 8238251
Accession: 008423998

The staining property of skin mast cells changed from Alcian blue+/berberine sulfate- to Alcian blue+/berberine sulfate+ in the skin of normal (+/+) and W-v/W-v mice. In contrast, this change did not occur in the skin of mi/mi mice. Heparin content and histamine content per a mi/mi skin mast cell were estimated to be 34% and 18% those of a +/+ skin mast cell, respectively. The low heparin content of mi/mi skin mast cells seemed to be consistent with the Alcian blue+/berberine sulfate - staining property. Expression of genes encoding mast cell-specific proteolytic enzymes was examined by Northern blotting and in situ hybridization. Messenger RNA of mast cell carboxypeptidase A was expressed most of all by +/+, W-v/ W-v, and mi/mi skin mast cells, but mRNA of mouse mast cell protease (MMCP)-6 was expressed by approximately a half of +/+ and W-v/W-v skin mast cells and by only 3% of mi/mi skin mast cells. A significant amount of MMCP-2 mRNA was not expressed in the skin of all +/+, W-v/W-v and mi/mi mice. This shows the presence of at least three phenotypes in skin mast cells of mice. berberine sulfate+/MMCP-6+, berberine sulfate+/MMCP-6+ and berberine sulfate-/MMCP-6-. The in situ hybridization of mRNA of mast cell-specific proteolytic enzymes seemed to be useful to describe abnormalities of mast cell differentiation in the skin of mi/mi mice.

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