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Definition of genetic determinants of nisin production ability in Lactococcus lactis subsp. lactis strains isolated from Turkey



Definition of genetic determinants of nisin production ability in Lactococcus lactis subsp. lactis strains isolated from Turkey



Turkish Journal of Biology 20(SUPPL ): 9-18



The abilities of 142 strains of Lactococcus lactis subsp. lactis isolated from Turkey to produce nisin were studied. Only four strains of the 142 were shown to produce nisin significantly. Mutagenesis studies involving the application of mutagenizing agents ethidium bromide, acriflavin, and ultraviolet light indicated that enhanced production of nisin in the four strains was not controlled by plasmids, but rather that nisin production was directed by elements in chromosomal DNA. All nisin producing lactococci were challenged with infection into 27 lactococcal phages. LL3 of these lactococcal strains showed particular sensitivity to infection by P90-60 phage. None of these nisin-producing strains harbored a prophage.

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Accession: 008424428

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