+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Detection of foot-and-mouth disease by reverse transcription polymerase chain reaction and virus isolation in contact sheep without clinical signs of foot-and-mouth disease



Detection of foot-and-mouth disease by reverse transcription polymerase chain reaction and virus isolation in contact sheep without clinical signs of foot-and-mouth disease



Veterinary Quarterly 20 Suppl 2: S37-S40



Two non-vaccinated sheep were experimentally infected with FMDV and one day later 4 other sheep were brought in contact. Although the contact sheep showed no clinical signs, serology indicated that all sheep became infected. Various secretion samples, taken over a period of at least one month, and various tissue samples were examined for the presence of FMDV by RT-PCR and by virus isolation. FMDV was most often found in saliva (mouth swabs), followed by nasal secretion and sera. Faecal material, wool and milk were less suitable. The period of detection with the highest frequency of positive isolations was between 2 to 4 days pi for the infected sheep and between 5 to 10 days pc for the contact animals. It was established that in subclinically infected sheep, with a very low amount of virus present, FMD viral RNA could be detected by a sensitive RT-PCR-ELISA although virus isolation and standard RT-PCR remained negative. Moreover there was some evidence of active spreading of FMDV from the contact sheep to two sentinel pigs. This indicates that serologically positive contact sheep without clinical signs may be considered as a danger for the transmission of FMDV.

(PDF emailed within 0-6 h: $19.90)

Accession: 008439627

Download citation: RISBibTeXText

PMID: 9652065

DOI: 10.1080/01652176.1998.9694964



Related references

Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus. Journal of Veterinary Science 6(3): 207-212, 2005

Validation of a LightCycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus. Journal of Virological Methods 113(1): 35-41, 2003

Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay. Journal of Virological Methods 105(1): 67-80, 2002

Detection of foot and mouth disease virus by real-time, fluorogenic TaqMan reverse transcription polymerase chain reaction assay. Chinese Journal of Veterinary Science 25(2): 116-119, 2005

Comparison of reverse transcription polymerase chain reaction, enzyme linked immunosorbent assay and virus isolation for the routine diagnosis of foot-and-mouth disease. Journal of Virological Methods 70(2): 213-217, 1998

Pan-serotypic detection of foot-and-mouth disease virus using a minor groove binder probe reverse transcription polymerase chain reaction assay. Journal of Virological Methods 174(1-2): 117-119, 2011

Detection of foot-and-mouth disease virus: comparative diagnostic sensitivity of two independent real-time reverse transcription-polymerase chain reaction assays. Journal of Veterinary Diagnostic Investigation 18(1): 93-97, 2006

Performance of real-time reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus during field outbreaks in the United Kingdom in 2007. Journal of Veterinary Diagnostic Investigation 21(3): 321-330, 2009

Detection of foot-and-mouth disease virus in milk samples by real-time reverse transcription polymerase chain reaction: Optimisation and evaluation of a high-throughput screening method with potential for disease surveillance. Veterinary Microbiology 223: 189-194, 2018

A reverse transcription polymerase chain reaction for measurement of foot and mouth disease virus in bovine oesophageal-pharyngeal secretions. Chinese Journal of Veterinary Science and Technology 26(8): 3-4, 1996

Performance of a foot-and-mouth disease virus reverse transcription-polymerase chain reaction with amplification controls between three real-time instruments. Journal of Veterinary Diagnostic Investigation 19(1): 9-20, 2007

Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction. Journal of Virological Methods 89(1/2): 167-176, 2000

Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus. Journal of the American Veterinary Medical Association 220(11): 1636-1642, 2002

Use of the reverse transcription polymerase chain reaction (RT-PCR) for the rapid diagnosis of foot and mouth disease in South America. Veterinary Research Communications 27(1): 63-71, 2003

Differentiation of the seven serotypes of foot-and-mouth disease virus by reverse transcriptase polymerase chain reaction. Journal of Virological Methods 67(1): 35-44, 1997