+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of growth hormone, prolactin and human beta-1-chorionic gonadotropin mRNA in growth hormone-secreting pituitary adenomas and in prolactin-secreting pituitary adenomas by in situ hybridization using a non-isotopic detection method

Detection of growth hormone, prolactin and human beta-1-chorionic gonadotropin mRNA in growth hormone-secreting pituitary adenomas and in prolactin-secreting pituitary adenomas by in situ hybridization using a non-isotopic detection method

Acta Endocrinologica 128(5): 411-417

A non-isotopic in situ hybridization method with digoxigenin-labelled probes was used to examine growth hormone (GH), prolactin (PRL) and human beta-chorionic gonadotropin (beta-hCG(LH)) gene expression in 63 pituitary tumours in acromegaly and 20 adenomas in hyperprolactinaemia. hCG and LH were detected simultaneously because of the extensive homology (more than 90%) of their mRNA sequences (1). A comparison with former results obtained with 35S-labelled probes shows the value of the easier and faster non-isotopic method. Additionally, immunohistochemical data are included to give even more evidence for the synthesis of the respective hormones by the tumour cells. In all 63 adenomas in acromegaly, GH mRNA was revealed in 59 PRL mRNA and in 36 beta-hCG(LH) mRNA. A positive immunostaining for GH was found in all, for PRL in 40, and for beta-hCG(LH) in 34 adenomas. The comparison of the two in situ hybridization methods revealed no differences concerning GH mRNA detection, but not all tumours positive after non-isotopic PRL and beta-hCG (LH) mRNA detection showed signals with the radioactive method. Referring to the 20 PRL-secreting adenomas, PRL gene expression was demonstrable in all, GH mRNA in 12, and beta-hCG(LH) mRNA in 2 cases. Comparing the positive results of immunohistochemistry with those of in situ hybridization, correspondence was found in 19 cases for PRL, in 5 cases for GH and in no case for beta-hCG(LH).

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 008439805

Download citation: RISBibTeXText

PMID: 8317187

Related references

Detection of familial defective alolipo-protein B-100 among patients diagnosed with heterozygous familial hyper-cholesterolemia in Maritime Canada. Clinical Biochemistry 26(2): 127-128, 1993

Detection of growth hormone and growth hormone-releasing hormone-related messenger RNA in rat leukocytes by the polymerase chain reaction. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 198(1): 643-648, 1991

Detection of glycopeptide resistance in clinical isolates of Gram-positive bacteria. Journal of Antimicrobial ChemoTherapy 28(4): 483-486, 1991

Human growth hormone and insulin like growth factor 1 enhance the proliferation of human leukemic blasts. Journal of Clinical Oncology 9(3): 394-399, 1991

Clinical study of the antiarrhythmic action of Mexityl in ventricular disorders of the cardiac rhythm. Kardiologiia 19(10): 17-21, 1979

Basic fibroblastic growth factor in the urine of children with myelodysplasia A potential marker of bladder pathology. Pediatrics 98(3 Part 2): 615, 1996

Morphological studies on microtubules in neutrophils of patients with behcet's disease. Hokkaido Journal of Medical Science 63(1): 7-14, 1988

Somatostatin receptors in human colorectal cancer. European Journal of Surgical Oncology 18(1): 27-30, 1992

Prolactin function in erectile idiopathic impotence. Problemes Actuels d'Endocrinologie et de Nutrition 1977(21): 165-183, 1977

3H]Spiperone binding sites in brain: autoradiographic localization of multiple receptors. Brain Research 213(2): 277-289, 1981

Tissue kallikrein and kinins in mediating the renal hemodynamic responses to diabetes mellitus and dietary protein. Rifkin, H , J A Colwell And S I Taylor (Ed ) International Congress Series, 1000 Diabetes ; 14th International Diabetes Federation Congress: Washington, D C , Usa, June 23-28, Xxix+1337p Elsevier Science Publishers B V : Amsterdam, Netherlands; (Dist in The Usa And Canada By Elsevier Science Publishing Co , Inc : New York, New York, Usa) Illus Maps 184-188, 1991

Expression of p53, Bax and Bcl-2 proteins in hepatocytes in non-alcoholic fatty liver disease. World Journal of Gastroenterology 12(38): 6198-6202, 2006

Morphological studies on mitogen and antigen presentation by human and rabbit cells. Advances in Experimental Medicine and Biology 237: 843-848, 1988

Ultrastructural study of hydrangea leaf tissue infected by mycoplasma-like organisms. Phytopathologia Mediterranea 29(3): 168-174, 1990

Ultrastructural study of mitosis in the coenocytic soil alga Protosiphon botryoides (Chlorophyceae) with special reference to absolute configuration and behaviour of the basal apparatus. Cryptogamic Botany 3(2-3): 221-234, 1993