Detection of numerical chromosome aberrations in brain tumours by fluorescence in situ hybridization on smear preparations of small tumour biopsies

Löw, M.; Feiden, W.; Moringlane, J.R.; Zang, K.D.; Henn, W.

Neuropathology and Applied Neurobiology 20(5): 432-438

1994


ISSN/ISBN: 0305-1846
PMID: 7845529
DOI: 10.1111/j.1365-2990.1994.tb00993.x
Accession: 008441091

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Abstract
We present a technique for the preparation and interphase cytogenetic analysis of native tumour cell nuclei from smear preparations of small biopsies of human brain tumours. The presence of tumour-specific numerical chromosome aberrations can be determined by fluorescence in situ hybridization (FISH) analysis using chromosome-specific repetitive DNA probes. The FISH analysis of smear preparations provides cytogenetic information about brain tumour samples, within 2 days, avoiding time-consuming and artefact-prone tumour cell culture.

Detection of numerical chromosome aberrations in brain tumours by fluorescence in situ hybridization on smear preparations of small tumour biopsies