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Detection of the eaeA gene in Escherichia coli isolated from children with diarrhoea and characterization of the strains possessing the eaeA gene



Detection of the eaeA gene in Escherichia coli isolated from children with diarrhoea and characterization of the strains possessing the eaeA gene



Zhonghua Weishengwuxue He Mianyixue Zazhi 17(4): 305-308



Two hundred and twenty one strains of Escherichia coli of 37 serogroups isolated from children with diarrhoea in Kenya were examined for the eaeA gene, which encodes the expression of the attaching and effacing lesions of Escherichia coli, by using an alkaline phosphatase conjugated oligonucleotide probe. The strains positive for eaeA gene were further assayed for bfpA and slt genes, and also for HEp-2 adhesion and fluorescent actin staining (FAS) tests. The results showed that the eaeA prevalence rate was 19.5%, with those in EPEC, EHEC and other serogroups being 31.6%, 66.7% and 8.9%, respectively. Based on the probes for bfpA and slt genes, HEp-2 adhesion and FAS tests, 5 virulence patterns were differentiated among eaeA-harboring E. coli. This indicates that eaeA gene is not limited to EPEC and EHEC, that the eaeA-harboring E. coli are heterogeneous with respect to their virulence determinants.

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